Fig. 6
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- ZDB-FIG-241016-62
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- Lascaux et al., 2024 - TEX264 drives selective autophagy of DNA lesions to promote DNA repair and cell survival
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TEX264 is the receptor for TOP1cc degradation by selective autophagy (A) Overlap of known autophagy receptors at replication fork by NCC-SILAC-MS63 and in lysosomes upon 50 nM CPT by LysoIP-MS. (B) AlphaFold prediction of TEX264 structure, with indicated domains. (C) Immunofluorescence after 2 h of recovery after 2 h of 50 nM CPT and quantification (n = 3). Two-way ANOVA. Error bar, SD. (D) LysoIP performed after 6 h of 50 nM CPT in TEX264KO transiently expressing TEX264-WT or indicated TEX264 variants (n = 3). (E) Clonogenic assay after 8 h of CPT (n = 3). Error bar, SEM. (F) Proximity-ligation assay (PLA) between TEX264-V5-WT and LC3-GFP after 30 min of 50 nM CPT (n = 3) and quantification. Control 1, no LC3-GFP transfection; control 2, GFP antibody only (no V5); control 3, V5 antibody only (no GFP). Two-way ANOVA. Error bar, SD. Scale bar, 10 μm. ∗p < 0.05; ∗∗p < 0.005; ∗∗∗p < 0.0005; ns, not significant. See also Figure S6 and Table S3. |
Reprinted from Cell, 187(20), Lascaux, P., Hoslett, G., Tribble, S., Trugenberger, C., Antičević, I., Otten, C., Torrecilla, I., Koukouravas, S., Zhao, Y., Yang, H., Aljarbou, F., Ruggiano, A., Song, W., Peron, C., Deangeli, G., Domingo, E., Bancroft, J., Carrique, L., Johnson, E., Vendrell, I., Fischer, R., Ng, A.W.T., Ngeow, J., D'Angiolella, V., Raimundo, N., Maughan, T., Popović, M., Milošević, I., Ramadan, K., TEX264 drives selective autophagy of DNA lesions to promote DNA repair and cell survival, 5698-5718.e26, Copyright (2024) with permission from Elsevier. Full text @ Cell