Fig. 5
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- ZDB-FIG-241016-60
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- Lascaux et al., 2024 - TEX264 drives selective autophagy of DNA lesions to promote DNA repair and cell survival
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Lysosomal degradation of TOP1cc occurs in interphase cells through the nuclear envelope (A) LysoIP performed after 6 h of 50 nM CPT in unsynchronized or S-phase HeLa cells (n = 3). SE, short exposure; LE, long exposure. (B) Live-cell imaging started 3 min after treatment with 50 nM CPT. Normalized fluorescence intensity measured along the dashed line for both mCherry and GFP. Scale bar, 5 μm; zoom panel, 1 μm. (C) Live-cell imaging. Arrows pointing at the alteration of Lamin A/C-GFP integrity at lysosomal contact sites (n = 3). Rendering by TrackMate of lysosomes and nuclear envelope integrity. Scale bar, 10 μm. (D) Volume electron microscopy images with nuclei segmentation and three-dimensional (3D) rendering after 30 min of 50 nM CPT. INM, inner nuclear membrane; ONM, outer nuclear membrane; Nu, nucleoplasm; Cy, cytoplasm; A, autophagosome. Scale bar, 500 nm. Blister-positive cells present at least 2 enlarged spaces between the INM and the ONM of more than 150 nm, close to a membrane invagination region. (E) Cryo-focused ion beam (FIB) milling and cryo-fluorescence correlation using LysoTracker after 30 min of 50 nM CPT. (Top) Computational slice through a tomogram; (middle) segmentation of the tomogram; (bottom) overlay. Scale bar, 200 nm. See also Figure S5 and Videos S1, S2, S3, and S4. |
Reprinted from Cell, 187(20), Lascaux, P., Hoslett, G., Tribble, S., Trugenberger, C., Antičević, I., Otten, C., Torrecilla, I., Koukouravas, S., Zhao, Y., Yang, H., Aljarbou, F., Ruggiano, A., Song, W., Peron, C., Deangeli, G., Domingo, E., Bancroft, J., Carrique, L., Johnson, E., Vendrell, I., Fischer, R., Ng, A.W.T., Ngeow, J., D'Angiolella, V., Raimundo, N., Maughan, T., Popović, M., Milošević, I., Ramadan, K., TEX264 drives selective autophagy of DNA lesions to promote DNA repair and cell survival, 5698-5718.e26, Copyright (2024) with permission from Elsevier. Full text @ Cell