Fig. 7

Deletion of cd248a increases hypoxia-induced apoptosis.a Confocal micrograph showing pericytes (pdgfrβ+ cells, green) and TUNEL staining (red) in WT and cd248acc11/cc11 larvae with or without tp53 MO injection at 4 dpf. Arrows indicate TUNEL-positive pericytes. Scale bar = 50 μm. b Quantification of pericyte numbers in WT and cd248acc11/cc11 larvae with or without tp53 MO injection at 4 dpf within the imaging area (n = 8 larvae). c Quantification of the percentage of TUNEL-positive pericytes at 4 dpf represented as a ratio of the TUNEL-positive pericyte to the total number of pericytes within the imaging area (n = 8 larvae). The effectiveness of the tp53 MO in blocking transcription (d) and translation (e) (n = 3 biologically independent experiments). f Relative expression levels of apoptosis-related genes in pericytes isolated from cd248a+/+ and cd248acc11/cc11 larvae with or without exposure to CoCl₂ (n = 3 biologically independent experiments). Data in all quantitative panels are presented as mean ± SD; two-tailed unpaired t test (d), and two-way ANOVA (b, c and f). *, p < 0.05. **, p < 0.01. ***, p < 0.001. ****, p < 0.0001. ns: no significance.