Fig. 3

Knockouts of cd248a and cd248b genes in zebrafish using CRISPR/Cas9 system.Diagrams depicting the complete gene structures and deletion mutants of cd248a (a) and cd248b (b). The sgRNA target sequence is underlined. The wild-type Cd248a protein consists of 719 amino acids; the Cd248a mutant (cd248acc11/cc11) consists of only 65 amino acids identical to the wild type and 13 frameshifted amino acids. The wild type Cd248b protein consists of 668 amino acids; the Cd248b mutant (cd248bcc12/cc12) consists of only 56 amino acids identical to wild type and 4 frameshifted amino acids. Sequencing analysis shows indels sites in cd248acc11/cc11 (c) and cd248bcc12/cc12 (d), indicated with the blue box. e Survival rate of cd248acc11/cc11, cd248bcc12/cc12 and wild type zebrafish from 1 to 5 dpf (n = 3 biologically independent experiments). f Body length of cd248acc11/cc11, cd248bcc12/cc12 and wild-type zebrafish from 2 to 6 dpf (n ≥ 12 embryos or larvae). g Representative images of cd248acc11/cc11, cd248bcc12/cc12 and wild-type larvae at 3 dpf and 5 dpf. Scale bars = 500 μm. Data were analyzed using two-way ANOVA and presented as mean ± SD. ns: no significance. Different alphabets (a and b) between groups indicate significant differences (p < 0.05).