PUBLICATION
Cd248a regulates pericyte development and viability in zebrafish
- Authors
- Wang, C., Zhong, Y., Zhang, Y., Jiang, Y., Wang, C., An, L., Luo, C., Yang, L., He, Z., Yang, Y., Luo, M., Mao, M., Wang, W., Liu, Q., Shi, Y., Ping, Y.F.
- ID
- ZDB-PUB-250318-14
- Date
- 2025
- Source
- Communications biology 8: 451451 (Journal)
- Registered Authors
- Keywords
- none
- MeSH Terms
-
- Blood-Brain Barrier/metabolism
- Gene Expression Regulation, Developmental
- Zebrafish*/embryology
- Zebrafish*/metabolism
- Pericytes*/drug effects
- Pericytes*/metabolism
- Cell Proliferation
- Zebrafish Proteins*/genetics
- Zebrafish Proteins*/metabolism
- Embryonic Development
- Animals
- Antigens, CD/genetics
- Antigens, CD/metabolism
- Apoptosis*
- PubMed
- 40097701 Full text @ Commun Biol
Citation
Wang, C., Zhong, Y., Zhang, Y., Jiang, Y., Wang, C., An, L., Luo, C., Yang, L., He, Z., Yang, Y., Luo, M., Mao, M., Wang, W., Liu, Q., Shi, Y., Ping, Y.F. (2025) Cd248a regulates pericyte development and viability in zebrafish. Communications biology. 8:451451.
Abstract
CD248 is a pericyte marker during embryonic and tumor neovascularization. Although its expression pattern and function in mammalian pericytes have been extensively studied, its role in zebrafish pericytes remains largely unexplored. In this study, we identify that among the two zebrafish orthologs of human CD248, cd248a, rather than cd248b, is predominantly expressed in pericytes during embryonic development. We generate cd248a and cd248b mutant zebrafish (cd248acc11/cc11 and cd248bcc12/cc12) and observe a significant reduction in pericyte numbers in cd248acc11/cc11 mutants, accompanied by disruption of the blood-brain barrier. Notably, treatment with AG1295, a platelet-derived growth factor receptor inhibitor, attenuates the increase in pericyte proliferation induced by cd248a overexpression. Additionally, we find that CoCl2-induced pericyte apoptosis is enhanced in cd248acc11/cc11 larvae, indicating that cd248a provides protection against hypoxia-induced apoptosis. Taken together, our findings elucidate the role and underlying mechanisms of cd248a in regulating pericyte proliferation and apoptosis in zebrafish.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping