Figure 10.

Grp156 was not required for pairing of pre- and post-synapses in lateral-line hair cells.

(A–C) Images neuromasts immunolabeled with Maguk and Ribeye b to stain HC post- and pre-synapses, respectively at 5 dpf. (A) Example of an immunostain of a control neuromast showing three complete synapses to the right. (B) Image of an emx2 mutant neuromast that shows complete synapses, as well as unpaired pre- and postsynapses, shown to the right. (C) Image of a gpr156 mutant neuromast, with complete synapses shown to the right. (D) Quantification revealed the same number of complete synapses per HC in emx2 and gpr156 mutants compared to controls. (E–F) In gpr156 mutants, quantification revealed there is no difference in the number of unpaired pre- or post-synapses per neuromast compared to controls. In emx2 mutants, there were significantly more pre- or post-synapses per neuromast compared to controls. SEM is shown in D–F. Scale bar = 5 µm, 0.5 µm for insets. Unpaired t-test were used to make comparisons. *p<0.05, ****p<0.0001.