Figure 3.

No effects of Gpr156 deletion on basolateral voltage-dependent conductances were detected in LES HCs.

(A–D) Exemplar voltage-dependent currents from lateral extrastriolar (LES) type I (A, C) and type II (B, D) HCs of Gpr156^del/+ (A, B) and Gpr156^del/del (C, D) utricles. There are significant differences between HC types, but these differences are seen in both Gpr156^del/+ (A, B) and Gpr156^del/del utricles (C, D). A pre-pulse from holding potential to –124 mV shows the usual type I-type II difference in K⁺ conductances at or near resting potential: the prepulse deactivates g_K,L in type I cells (A,C), and activates a small inwardly rectifying current in type II cells (B, D). Following the pre-pulse, test steps were iterated in 5 mV increments from –124 mV to –54 mV (type I) or from –124 mV to +6 mV (type II), activating currents with very different time course and voltage dependence. (E, F) G(V) relations measured from tail currents at the end of the iterated voltage steps show that g_K,L activated positive to –114 mV (E) while type II delayed rectifying currents activated positive to –64 mV (F).