Figure 9—figure supplement 1.

Gpr156 was not required for lateral-line afferents to select Emx2⁺ HCs.

(A–H) Images of a control neuromast innervated by a single afferent fiber that contacts Emx2⁺ cells. (A) Phalloidin label reveals 14 hair bundles (7 A to P and 7 P to A hair bundles). Z-stack projections show all HCs labeled with Myo7a (B) a single afferent fiber expressing tdTomato (C) and Emx2⁺ and Emx2^- HCs (D). (E–H) Selected optical sections of (B–D) highlight the single afferent contacting individual Emx2⁺ HCs. (I–P) Images of a gpr156 mutant neuromast innervated by a single afferent fiber that contacts Emx2⁺ cells. (I) Phalloidin label revealed 14 hair bundles (12 A to P and 1 P to A hair bundles, 1 at 90°). Z-stack projections show all HCs labeled with Myo7a (J) a single afferent fiber expressing tdTomato (K) and Emx2⁺ and Emx2^- HCs (L). M–O Selected optical sections of (J–L) highlight a single afferent in a gpr156 mutants contacting individual Emx2⁺ HCs. Arrows in A and I indicate the orientation of the hair bundles in each example. Scale bar: 5 µm.