PUBLICATION

Essential Role of Nr2f Nuclear Receptors in Patterning the Vertebrate Upper Jaw

Authors
Barske, L., Rataud, P., Behizad, K., Del Rio, L., Cox, S.G., Crump, J.G.
ID
ZDB-PUB-180124-2
Date
2018
Source
Developmental Cell   44(3): 337-347.e5 (Journal)
Registered Authors
Barske, Lindsey, Crump, Gage DeKoeyer
Keywords
COUP-TF, Endothelin1, Nr2f1, Nr2f2, Nr2f5, bone, cartilage, jaw, nuclear receptor, pharyngeal arches
Datasets
GEO:GSE101719
MeSH Terms
  • Body Patterning/physiology*
  • Zebrafish/embryology*
  • Zebrafish/physiology
  • Gene Expression Regulation, Developmental
  • DNA-Binding Proteins/genetics
  • DNA-Binding Proteins/metabolism*
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/metabolism*
  • Jaw/embryology*
  • Jaw/physiology
  • Endothelin-1/genetics
  • Endothelin-1/metabolism*
  • Maxilla/embryology*
  • Maxilla/physiology
  • Signal Transduction
  • Odontogenesis/physiology
  • Transcription Factors/genetics
  • Transcription Factors/metabolism*
  • Animals
  • Mutation
  • Chondrogenesis/physiology
(all 21)
PubMed
29358039 Full text @ Dev. Cell
Abstract
The jaw is central to the extensive variety of feeding and predatory behaviors across vertebrates. The bones of the lower but not upper jaw form around an early-developing cartilage template. Whereas Endothelin1 patterns the lower jaw, the factors that specify upper-jaw morphology remain elusive. Here, we identify Nuclear Receptor 2f genes (Nr2fs) as enriched in and required for upper-jaw formation in zebrafish. Combinatorial loss of Nr2fs transforms maxillary components of the upper jaw into lower-jaw-like structures. Conversely, nr2f5 misexpression disrupts lower-jaw development. Genome-wide analyses reveal that Nr2fs repress mandibular gene expression and early chondrogenesis in maxillary precursors. Rescue of lower-jaw defects in endothelin1 mutants by reducing Nr2f dosage further demonstrates that Nr2f expression must be suppressed for normal lower-jaw development. We propose that Nr2fs shape the upper jaw by protecting maxillary progenitors from early chondrogenesis, thus preserving cells for later osteogenesis.
Genes / Markers
Figures
Figure Gallery (11 images) / 2
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Expression
Phenotype
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
b1228TgTransgenic Insertion
    el2TgTransgenic Insertion
      el10TgTransgenic Insertion
        el159TgTransgenic Insertion
          el249TgTransgenic Insertion
            el506
              		Small Deletion
              el512
                		Small Deletion
                el523
                  		Small Deletion
                  el611
                    		Small Deletion
                    el647TgTransgenic Insertion
                      1 - 10 of 16
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                      Human Disease / Model
                      No data available
                      Sequence Targeting Reagents
                      Target Reagent Reagent Type
                      nr2f1bTALEN1-nr2f1bTALEN
                      nr2f2TALEN2-nr2f2TALEN
                      nr2f5TALEN1-nr2f5TALEN
                      1 - 3 of 3
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                      Fish
                      Fish
                      b1228Tg; el663Tg (TU)
                      dlx5aj1073aGt; el10Tg (TU)
                      edn1tf216b/tf216b; nr2f1bel523/el523; nr2f2el506/el506; nr2f5el611/el611 (TU)
                      edn1tf216b/tf216b; nr2f1bel523/el523; nr2f2el506/+; nr2f5el611/el611 (TU)
                      edn1tf216b/tf216b; nr2f1bel523/+; nr2f2el506/el506; nr2f5el611/el611 (TU)
                      edn1tf216b/tf216b (TU)
                      el10Tg (TU)
                      el159Tg; el662Tg (TU)
                      el249Tg; kca4Tg (TU)
                      el647Tg; el662Tg (TU)
                      1 - 10 of 18
                      Show
                      Antibodies
                      Name Type Antigen Genes Isotypes Host Organism
                      Ab1-sp7polyclonal
                        		IgGRabbit
                        Ab3-sox10polyclonalIgGRabbit
                        1 - 2 of 2
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                        Orthology
                        No data available
                        Engineered Foreign Genes
                        Marker Marker Type Name
                        CeruleanEFGCerulean
                        DsRedEFGDsRed
                        EGFPEFGEGFP
                        GAL4EFGGAL4
                        KikGREFGKikGR
                        1 - 5 of 5
                        Show
                        Mapping
                        No data available
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                        Citation
                        Barske, L., Rataud, P., Behizad, K., Del Rio, L., Cox, S.G., Crump, J.G. (2018) Essential Role of Nr2f Nuclear Receptors in Patterning the Vertebrate Upper Jaw. Developmental Cell. 44(3):337-347.e5.