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Early onset muscle and motor phenotypes in MZKOs. A, B: Analysis of locomotor function at 5 dpf showing significant reductions in distance (A) and average velocity (B) in MZKOs (***p < 0.001). C, D: Analysis of locomotor function between 7-14 dpf showing that MZKOs have significant reductions in maximum velocity (C) and acceleration (D) at all three time points compared to their MHet siblings but show an overall decline between 7-14 dpf (*p < 0.05, **p < 0.01, ***p < 0.001). E: Fluorescent staining of muscle at 7 dpf showing overall uniform muscle organization with a few detached fibers (left, arrowhead), normal laminin signal at the myotendinous junctions (middle), and detachments within the slow twitch fiber layer (right, asterisks) (20X magnification, scale bars: 100 µm). F: Fluorescent staining of muscle at 10 dpf showing overall deterioration of muscle fibers (left), diffuse and disrupted laminin signal at the myotendinous junctions (middle), and uniform degeneration of the slow twitch fiber layer (right) (20X magnification, scale bars: 100 µm).
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