PUBLICATION

Cilia Control Vascular Mural Cell Recruitment in Vertebrates

Authors
Chen, X., Gays, D., Milia, C., Santoro, M.M.
ID
ZDB-PUB-170126-1
Date
2017
Source
Cell Reports   18: 1033-1047 (Journal)
Registered Authors
Gays, Dafne, Santoro, Massimo
Keywords
CRISPR-Cas9, blood flow, cilia, mural cells, zebrafish model
MeSH Terms
  • Receptors, Notch/antagonists & inhibitors
  • Receptors, Notch/genetics
  • Receptors, Notch/metabolism
  • Zebrafish/growth & development
  • Zebrafish/metabolism
  • Troponin T/antagonists & inhibitors
  • Troponin T/genetics
  • Troponin T/metabolism
  • Shear Strength
  • Embryo, Nonmammalian/cytology
  • Embryo, Nonmammalian/drug effects
  • Embryo, Nonmammalian/metabolism
  • Endothelial Cells/cytology
  • Endothelial Cells/metabolism*
  • Clustered Regularly Interspaced Short Palindromic Repeats/genetics
  • RNA Interference
  • Signal Transduction/drug effects
  • Animals, Genetically Modified/genetics
  • Animals, Genetically Modified/growth & development
  • Animals, Genetically Modified/metabolism
  • Zebrafish Proteins/antagonists & inhibitors
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/metabolism*
  • Forkhead Transcription Factors/genetics
  • Forkhead Transcription Factors/metabolism*
  • Hemodynamics
  • Blood Flow Velocity
  • Quinazolinones/pharmacology
  • Cilia/metabolism*
  • Morpholinos/genetics
  • Morpholinos/metabolism
  • Animals
(all 32)
PubMed
28122229 Full text @ Cell Rep.
Abstract
Vascular mural cells (vMCs) are essential components of the vertebrate vascular system, controlling blood vessel maturation and homeostasis. Discrete molecular mechanisms have been associated with vMC development and differentiation. The function of hemodynamic forces in controlling vMC recruitment is unclear. Using transgenic lines marking developing vMCs in zebrafish embryos, we find that vMCs are recruited by arterial-fated vessels and that the process is flow dependent. We take advantage of tissue-specific CRISPR gene targeting to demonstrate that hemodynamic-dependent Notch activation and the ensuing arterial genetic program is driven by endothelial primary cilia. We also identify zebrafish foxc1b as a cilia-dependent Notch-specific target that is required within endothelial cells to drive vMC recruitment. In summary, we have identified a hemodynamic-dependent mechanism in the developing vasculature that controls vMC recruitment.
Genes / Markers
Figures
Figure Gallery (16 images) / 2
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Expression
Phenotype
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
fh332
    Point Mutation
    hu10049TgTransgenic Insertion
      kca3TgTransgenic Insertion
        nz101TgTransgenic Insertion
          s843TgTransgenic Insertion
            sd2TgTransgenic Insertion
              um14TgTransgenic Insertion
                uto2TgTransgenic Insertion
                  uto5TgTransgenic Insertion
                    uto37TgTransgenic Insertion
                      1 - 10 of 11
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                      Human Disease / Model
                      No data available
                      Sequence Targeting Reagents
                      1 - 10 of 10
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                      Fish
                      Antibodies
                      No data available
                      Orthology
                      No data available
                      Engineered Foreign Genes
                      Marker Marker Type Name
                      DsRedEFGDsRed
                      EGFPEFGEGFP
                      GAL4FFEFGGAL4FF
                      mCherryEFGmCherry
                      1 - 4 of 4
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                      Mapping
                      No data available