Gene
hey2
- ID
- ZDB-GENE-000526-1
- Name
- hes-related family bHLH transcription factor with YRPW motif 2
- Symbol
- hey2 Nomenclature History
- Previous Names
- Type
- protein_coding_gene
- Location
- Chr: 20 Mapping Details/Browsers
- Description
- Predicted to enable DNA-binding transcription factor activity, RNA polymerase II-specific and RNA polymerase II cis-regulatory region sequence-specific DNA binding activity. Acts upstream of or within with a positive effect on cardiac muscle cell proliferation. Acts upstream of or within cardiac muscle tissue regeneration; circulatory system development; and negative regulation of DNA-templated transcription. Predicted to be active in nucleus. Is expressed in several structures, including alar plate midbrain region; cardiovascular system; hematopoietic system; mesoderm; and nervous system. Orthologous to human HEY2 (hes related family bHLH transcription factor with YRPW motif 2).
- Genome Resources
- Note
- None
- Comparative Information
-
- All Expression Data
- 76 figures from 62 publications
- Cross-Species Comparison
- High Throughput Data
- Thisse Expression Data
-
- eu521 (19 images)
Wild Type Expression Summary
- All Phenotype Data
- 17 figures from 10 publications
- Cross-Species Comparison
- Alliance
Phenotype Summary
Mutations
| Allele | Type | Localization | Consequence | Mutagen | Supplier |
|---|---|---|---|---|---|
| hsc25 | Allele with one deletion | Exon 2 | Unknown | CRISPR | |
| hsc27Tg | Transgenic insertion | Unknown | Unknown | DNA and CRISPR | |
| ihb463 | Allele with one insertion | Exon 4 | Unknown | CRISPR | |
| ihb464 | Allele with one deletion | Exon 4 | Unknown | CRISPR | |
| m145 | Allele with one point mutation | Unknown | Missense | ENU | |
| sa10785 | Allele with one point mutation | Unknown | Splice Site | ENU | |
| sa16273 | Allele with one point mutation | Unknown | Premature Stop | ENU | |
| sa23783 | Allele with one point mutation | Unknown | Premature Stop | ENU | |
| sa29430 | Allele with one point mutation | Unknown | Premature Stop | ENU | |
| um216 | Allele with one deletion | Unknown | Frameshift, Premature Stop | CRISPR |
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| Targeting Reagent | Created Alleles | Citations |
|---|---|---|
| CRISPR1-hey2 | Gibb et al., 2018 | |
| CRISPR2-hey2 | Shin et al., 2019 | |
| CRISPR3-hey2 | Zebrafish Nomenclature Committee | |
| CRISPR4-hey2 | (2) | |
| CRISPR5-hey2 | Ghanta et al., 2021 | |
| MO1-hey2 | N/A | (11) |
| MO2-hey2 | N/A | Gibb et al., 2018 |
| TALEN1-hey2 | (2) | |
| TALEN2-hey2 | Hermkens et al., 2015 |
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Human Disease
Domain, Family, and Site Summary
Domain Details Per Protein
| Protein | Additional Resources | Length | Hairy and Enhancer of Split/HEY-related | Helix-loop-helix DNA-binding domain superfamily | Myc-type, basic helix-loop-helix (bHLH) domain | Orange domain |
|---|---|---|---|---|---|---|
| UniProtKB:Q9I9L0 | InterPro | 324 |
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Interactions and Pathways
No data available
Plasmids
| Construct | Regulatory Region | Coding Sequence | Species | Tg Lines | Citations |
|---|---|---|---|---|---|
| Tg1(FRT-Xla.Actc1:DsRed-GAB-FRT,LOXP-hey2-LOXP-gata2a:EGFP-5HS4) |
|
| 3 | (3) | |
| Tg2(FRT-Xla.Actc1:DsRed-GAB-FRT,LOXP-hey2-LOXP-gata2a:EGFP-5HS4) |
|
| 3 | (3) | |
| Tg(hey2:EGFP) |
|
| 1 | She et al., 2020 | |
| Tg(hey2:mCherry) |
|
| 1 | She et al., 2020 | |
| Tg(myl7:LOXP-NLS-mCherry-LOXP-hey2-EGFP) |
| 1 | (2) |
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| Relationship | Marker Type | Marker | Accession Numbers | Citations |
|---|---|---|---|---|
| Contained in | BAC | CH211-172B22 | ZFIN Curated Data | |
| Encodes | EST | eu521 | Thisse et al., 2005 | |
| Encodes | cDNA | MGC:136746 | ZFIN Curated Data | |
| Encodes | cDNA | MGC:194026 | ZFIN Curated Data | |
| Encodes | cDNA | MGC:194033 | ZFIN Curated Data |
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| Type | Accession # | Sequence | Length (nt/aa) | Analysis |
|---|---|---|---|---|
| RNA | RefSeq:NM_131622 (1) | 1898 nt | ||
| Genomic | GenBank:CT573083 (1) | 40018 nt | ||
| Polypeptide | UniProtKB:Q9I9L0 (1) | 324 aa |
- She, P., Gao, B., Li, D., Wu, C., Zhu, X., He, Y., Mo, F., Qi, Y., Jin, D., Chen, Y., Zhao, X., Lin, J., Hu, H., Li, J., Zhang, B., Xie, P., Lin, C., Christoffels, V.M., Wu, Y., Zhu, P., Zhong, T.P. (2025) The transcriptional repressor HEY2 regulates mitochondrial oxidative respiration to maintain cardiac homeostasis. Nature communications. 16:232232
- Carey, C.M., Hollins, H.L., Schmid, A.V., Gagnon, J.A. (2024) Distinct features of the regenerating heart uncovered through comparative single-cell profiling. Biology Open. 13(4):
- da Silva, A.R., Gunawan, F., Boezio, G.L.M., Faure, E., Théron, A., Avierinos, J.F., Lim, S., Jha, S.G., Ramadass, R., Guenther, S., Looso, M., Zaffran, S., Juan, T., Stainier, D.Y.R. (2024) egr3 is a mechanosensitive transcription factor gene required for cardiac valve morphogenesis. Science advances. 10:eadl0633
- Duan, W., Huang, G., Sui, Y., Wang, K., Yu, Y., Chu, X., Cao, X., Chen, L., Liu, J., Eichler, E.E., Xiong, B. (2024) Deficiency of DDX3X results in neurogenesis defects and abnormal behaviors via dysfunction of the Notch signaling. Proceedings of the National Academy of Sciences of the United States of America. 121:e2404173121e2404173121
- Wang, A., Wan, X., Zhu, F., Liu, H., Song, X., Huang, Y., Zhu, L., Ao, Y., Zeng, J., Wang, B., Wu, Y., Xu, Z., Wang, J., Yao, W., Li, H., Zhuang, P., Jiao, J., Zhang, Y. (2024) Habitual Daily Intake of Fried Foods Raises Transgenerational Inheritance Risk of Heart Failure Through NOTCH1-Triggered Apoptosis. Research (Washington, D.C.). 7:04010401
- Greysson-Wong, J., Rode, R., Ryu, J.R., Chan, J.L., Davari, P., Rinker, K.D., Childs, S.J. (2023) rasa1-related arteriovenous malformation is driven by aberrant venous signalling. Development (Cambridge, England). 150(18):
- Heng, J., Shi, B., Zhou, J.Y., Zhang, Y., Ma, D., Yang, Y.G., Liu, F. (2023) Cpeb1b-mediated cytoplasmic polyadenylation of shha mRNA modulates zebrafish definitive hematopoiesis. Proceedings of the National Academy of Sciences of the United States of America. 120:e2212212120e2212212120
- Kondo, M., Kimura, K., Kobayashi-Sun, J., Yamamori, S., Taniguchi, M., Traver, D., Kobayashi, I. (2023) The sinusoidal hematopoietic niche is formed by Jam1a via Notch signaling in the zebrafish kidney. iScience. 26:106508106508
- Zhou, L., Zhang, W., Xiang, Y., Qian, Z., Zhou, J., Ni, L., Feng, Y., Gao, B. (2023) The apatinib and pemetrexed combination has antitumor and antiangiogenic effects against NSCLC. Open life sciences. 18:2022053320220533
- Li, Y., Tang, C., Liu, F., Zhu, C., Liu, F., Zhu, P., Wang, L. (2022) DNA methylation safeguards the generation of hematopoietic stem and progenitor cells by repression of Notch signaling. Development (Cambridge, England). 149(10):
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