PUBLICATION

BMP and non-canonical Wnt signaling are required for inhibition of secondary tail formation in zebrafish

Authors
Yang, Y., and Thorpe, C.
ID
ZDB-PUB-110603-16
Date
2011
Source
Development (Cambridge, England)   138(12): 2601-2611 (Journal)
Registered Authors
Thorpe, Chris
Keywords
zebrafish, BMP, wnt, tailbud, notochord, morphogenesis
MeSH Terms
  • Bone Morphogenetic Proteins/metabolism*
  • Animals
  • Tail/growth & development*
  • Morphogenesis*
  • Signal Transduction/physiology*
  • Wnt Proteins/metabolism*
  • Zebrafish/embryology*
  • Zebrafish/metabolism*
  • Body Patterning
(all 9)
PubMed
21610036 Full text @ Development
Abstract
The role of bone morphogenetic protein (BMP) signaling in specifying cell fate in the zebrafish tailbud has been well established. In addition to a loss of ventral tissues, such as ventral tailfin and cloaca, some embryos with compromised BMP signaling produce an additional phenotype: a ventrally located secondary tail containing both somitic muscle and notochord. This phenotype has been proposed to reflect a fate-patterning defect due to a change in a hypothesized BMP activity gradient. Here, we show that a defect in morphogenetic movements, not fate patterning, underlies the formation of secondary tails in BMP-inhibited embryos. Our data indicate that BMP signaling is activated in the ventroposterior tailbud to promote cell migration during tailbud protrusion, and that defective migration of these cells in BMP mutants ultimately leads to bifurcation of the caudal notochord. Additionally, we show that non-canonical Wnt signaling is also required for proper tail morphogenesis, possibly by maintaining cohesion of notochord progenitors by regulation of cadherin localization. We propose a model in which BMP and the non-canonical Wnt pathway regulate tail morphogenesis by controlling cell migration and cell adhesion within the tailbud.
Genes / Markers
Figures
Figure Gallery (13 images) / 2
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Expression
Phenotype
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
c161TgTransgenic Insertion
    dty40
      Point Mutation
      fr6
        Point Mutation
        m169
          Point Mutation
          tc263a
            Point Mutation
            ty68a
              Point Mutation
              1 - 6 of 6
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              Human Disease / Model
              No data available
              Sequence Targeting Reagents
              Target Reagent Reagent Type
              cdh2MO1-cdh2MRPHLNO
              dvl1bMO2-dvl1bMRPHLNO
              dvl2MO1-dvl2MRPHLNO
              dvl3aMO1-dvl3aMRPHLNO
              tll1MO2-tll1MRPHLNO
              1 - 5 of 5
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              Fish
              Antibodies
              Name Type Antigen Genes Isotypes Host Organism
              Ab1-pan-CadherinpolyclonalRabbit
              Ab1-smadpolyclonalIgGRabbit
              Ab2-ctnnbmonoclonalIgG1Mouse
              1 - 3 of 3
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              Orthology
              No data available
              Engineered Foreign Genes
              Marker Marker Type Name
              EGFPEFGEGFP
              1 - 1 of 1
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              Mapping
              No data available