PUBLICATION

Exocrine pancreas development in zebrafish

Authors
Yee, N.S., Lorent, K., and Pack, M.
ID
ZDB-PUB-050623-3
Date
2005
Source
Developmental Biology   284(1): 84-101 (Journal)
Registered Authors
Lorent, Kristin, Pack, Michael, Yee, Nelson S.
Keywords
Exocrine pancreas; Zebrafish; Development; Duct; Acinus; Jagged; Notch
MeSH Terms
  • Zebrafish/embryology*
  • Endoderm/physiology
  • In Situ Hybridization
  • Animals
  • Immunohistochemistry
  • Zebrafish Proteins/genetics
  • Morphogenesis/physiology*
  • Ubiquitin-Protein Ligases/genetics
  • Membrane Proteins/genetics
  • Models, Biological*
  • Mutagenesis
  • Microscopy, Electron, Transmission
  • Calcium-Binding Proteins/genetics
  • Cell Differentiation/physiology*
  • Pancreas/embryology*
  • Pancreas/ultrastructure
  • Intercellular Signaling Peptides and Proteins
  • Signal Transduction/physiology*
(all 18)
PubMed
15963491 Full text @ Dev. Biol.
Abstract
Although many of the genes that regulate development of the endocrine pancreas have been identified, comparatively little is known about how the exocrine pancreas forms. Previous studies have shown that exocrine pancreas development may be modeled in zebrafish. However, the timing and mechanism of acinar and ductal differentiation and morphogenesis have not been described. Here, we characterize zebrafish exocrine pancreas development in wild type and mutant larvae using histological, immunohistochemical and ultrastructural analyses. These data allow us to identify two stages of zebrafish exocrine development. During the first stage, the exocrine anlage forms from rostral endodermal cells. During the second stage, protodifferentiated progenitor cells undergo terminal differentiation followed by acinar gland and duct morphogenesis. Immunohistochemical analyses support a model in which the intrapancreatic ductal system develops from progenitors that join to form a contiguous network rather than by branching morphogenesis of the pancreatic epithelium, as described for mammals. Contemporaneous appearance of acinar glands and ducts in developing larvae and their disruption in pancreatic mutants suggest that common molecular pathways may regulate gland and duct morphogenesis and differentiation of their constituent cells. By contrast, analyses of mind bomb mutants and jagged morpholino-injected larvae suggest that Notch signaling principally regulates ductal differentiation of bipotential exocrine progenitors.
Genes / Markers
Figures
Figure Gallery (13 images) / 2
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Expression
Phenotype
No data available
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
kca3TgTransgenic Insertion
    kca4TgTransgenic Insertion
      m74
        Point Mutation
        m132
          Point Mutation
          m497
            Unknown
            p13cv
              Unknown
              p14nb
                Point Mutation
                p26nb
                  Unknown
                  p75fm
                    Unknown
                    p82mf
                      Unknown
                      1 - 10 of 12
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                      Human Disease / Model
                      No data available
                      Sequence Targeting Reagents
                      Target Reagent Reagent Type
                      jag1bMO1-jag1bMRPHLNO
                      jag2bMO1-jag2bMRPHLNO
                      1 - 2 of 2
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                      Fish
                      Antibodies
                      No data available
                      Orthology
                      No data available
                      Engineered Foreign Genes
                      No data available
                      Mapping
                      No data available