PUBLICATION

Exocrine pancreas development in zebrafish

Authors
Yee, N.S., Lorent, K., and Pack, M.
ID
ZDB-PUB-050623-3
Date
2005
Source
Developmental Biology   284(1): 84-101 (Journal)
Registered Authors
Lorent, Kristin, Pack, Michael, Yee, Nelson S.
Keywords
Exocrine pancreas; Zebrafish; Development; Duct; Acinus; Jagged; Notch
MeSH Terms
  • Signal Transduction/physiology*
  • In Situ Hybridization
  • Calcium-Binding Proteins/genetics
  • Zebrafish/embryology*
  • Mutagenesis
  • Cell Differentiation/physiology*
  • Endoderm/physiology
  • Membrane Proteins/genetics
  • Morphogenesis/physiology*
  • Models, Biological*
  • Animals
  • Ubiquitin-Protein Ligases/genetics
  • Pancreas/embryology*
  • Pancreas/ultrastructure
  • Zebrafish Proteins/genetics
  • Intercellular Signaling Peptides and Proteins
  • Immunohistochemistry
  • Microscopy, Electron, Transmission
(all 18)
PubMed
15963491 Full text @ Dev. Biol.
Abstract
Although many of the genes that regulate development of the endocrine pancreas have been identified, comparatively little is known about how the exocrine pancreas forms. Previous studies have shown that exocrine pancreas development may be modeled in zebrafish. However, the timing and mechanism of acinar and ductal differentiation and morphogenesis have not been described. Here, we characterize zebrafish exocrine pancreas development in wild type and mutant larvae using histological, immunohistochemical and ultrastructural analyses. These data allow us to identify two stages of zebrafish exocrine development. During the first stage, the exocrine anlage forms from rostral endodermal cells. During the second stage, protodifferentiated progenitor cells undergo terminal differentiation followed by acinar gland and duct morphogenesis. Immunohistochemical analyses support a model in which the intrapancreatic ductal system develops from progenitors that join to form a contiguous network rather than by branching morphogenesis of the pancreatic epithelium, as described for mammals. Contemporaneous appearance of acinar glands and ducts in developing larvae and their disruption in pancreatic mutants suggest that common molecular pathways may regulate gland and duct morphogenesis and differentiation of their constituent cells. By contrast, analyses of mind bomb mutants and jagged morpholino-injected larvae suggest that Notch signaling principally regulates ductal differentiation of bipotential exocrine progenitors.
Genes / Markers
Figures
Figure Gallery (13 images) / 2
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Expression
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay Figure
cpa5ABstandard conditionsPec-finIHC
cpa5ABstandard conditionsProtruding-mouth to Day 5IHC
cpa5ABchemical treatment: ethanolDay 4IHC
cpa5ABstandard conditionsDay 4IHC
cpa5ABstandard conditionsDay 4IHC
cpa5ABstandard conditionsDay 5IHC
cpa5ABstandard conditionsLong-pecNot DetectedIHC
cpa5ABchemical treatment: ethanolDay 4Not DetectedIHC
cpa5ABstandard conditionsDay 5IHC
cpa5AB + MO1-jag1b + MO1-jag2bstandard conditionsDay 5IHC
1 - 10 of 52
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Phenotype
No data available
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
kca3TgTransgenic Insertion
    kca4TgTransgenic Insertion
      m74
        		Point Mutation
        m132
          		Point Mutation
          m497
            		Unknown
            p13cv
              		Unknown
              p14nb
                		Point Mutation
                p26nb
                  		Unknown
                  p75fm
                    		Unknown
                    p82mf
                      		Unknown
                      1 - 10 of 12
                      Show
                      Human Disease / Model
                      No data available
                      Sequence Targeting Reagents
                      Target Reagent Reagent Type
                      jag1bMO1-jag1bMRPHLNO
                      jag2bMO1-jag2bMRPHLNO
                      1 - 2 of 2
                      Show
                      Fish
                      Fish
                      AB
                      AB + MO1-jag1b + MO1-jag2b
                      ahctf1ti262c/ti262c
                      ahcyp14nb
                      djmp26nb
                      kca3Tg
                      kca4Tg
                      mib1m132/m132 (AB)
                      mmsp13cv
                      piem497 (AB)
                      1 - 10 of 14
                      Show
                      Antibodies
                      No data available
                      Orthology
                      No data available
                      Engineered Foreign Genes
                      No data available
                      Mapping
                      No data available
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                      Citation
                      Yee, N.S., Lorent, K., and Pack, M. (2005) Exocrine pancreas development in zebrafish. Developmental Biology. 284(1):84-101.