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Fig. 5

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ZDB-FIG-250423-48

Publication

Wang et al., 2025 - Wdr5-mediated H3K4 methylation facilitates HSPC development via maintenance of genomic stability in zebrafish

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Fig. 5

Wdr5-mediated H3K4me3 maintains the expression of DNA repair genes in the CHT HSPCs. (A) Profile plots of H3K4me3 enrichment at promoter-marked regions and heatmap of H3K4me3 enrichment surrounding gene promoters in the CD41+gata1− HSPCs of CHT region from siblings and wdr5 mutants. (B) Bar plot showing the genomic distribution of H3K4me3 peaks in the CD41+gata1− HSPCs of CHT region from siblings and wdr5 mutants. (C) GO analysis for genes with reduced H3K4me3 peaks and expression level in the CD41+gata1− HSPCs of CHT region from wdr5 mutants. (D) qRT-PCR showing the expression of uvssa, ercc2, tonsl, brip1, and mlh1 in the CHT region of siblings, wdr5 mutants, wdr5 mutant× Tg(hsp70:flag-wdr5WT-EGFP) and wdr5 mutant× Tg(hsp70:flag-wdr5MF-EGFP) embryos at 3.5 dpf. wdr5WT and wdr5MF driven by hsp70 promoter were induced by heat shock at 28 hpf. Error bar, mean ± SD, n.s.: no significance, *P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001, ****P ≤ 0.0001.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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