FIGURE

Figure 4

ID
ZDB-FIG-250325-6
Publication
Zhang et al., 2025 - The mediator subunit complex protein MED15 promotes lipid deposition and cancer progression during hypoxia
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Figure 4

MED15 depletion suppresses the proliferation of tumor cells in vitro.AD, colony formation assay of WT and MED15 KO HCT116 cells (KO #17, KO #19) and 786-O cells (KO #5, KO #6). Cells were cultured for 9 days. Representative images from three independent experiments are shown in (A and C). Quantification of colony numbers is shown in (B and D). Data are represented as means ± SD. n = 3 biologically independent repeats. ∗∗∗p < 0.001 by one-way ANOVA with Sidak's t test. E and F, cell proliferation curves of WT and MED15 KO HCT116 cells (KO #17, KO #19) and 786-O cells (KO #5, KO #6). Cell numbers were determined by cell counter. Data are represented as means ± SD. n = 3 biologically independent extracts. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, ∗∗∗∗p < 0.0001 by one-way ANOVA with Sidak's t test. GJ, flow cytometry analysis of cell cycle distribution in WT and MED15 KO HCT116 cells (KO #17, KO #19) and 786-O cells (KO #5, KO #6). Representative images from three independent experiments are shown in (G and I). Statistics of cell cycle distribution are shown in (H and J). Data are represented as means ± SD. n = 3 biologically independent extracts. ∗∗∗p < 0.001, ∗∗∗∗p < 0.0001 by one-way ANOVA with Sidak's t test. ns, not significant; MED15, Mediator complex subunit 15.

Expression Data

Expression Detail
Antibody Labeling
Phenotype Data

Phenotype Detail
Acknowledgments
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