Fig. 3

Rotenone impairs dopamine synthesis and transport and induces accumulation of ɑ-synuclein in developing zebrafish embryos. Wild-type and Tg(huC:zsgreen-p2a-human a-synuclein) aged 7–8 h post fertilization (hpf) embryos were exposed with 30 nM rotenone to 72 hpf. (A) Embedded Specimen orientation. A: anterior, P: posterior, L: left, R: right. Analyzing the gate area indicated by the red dotted line. Scale bar: 10 µm. Tyrosine hydroxylase (TH, th) (B) mRNA expression, Scale bar: 100 µm, (C) representative images of immunohistochemistry, and (D) quantified of TH immunohistochemistry. A: anterior, P: posterior, L: left, R: right. N = 13–20. Scale bar: 10 µm. (E) dopamine transporter (DAT/slc6a3) mRNA expressions. N = 16–20. (F) Representative images of the head area and (G) measured the area of red dotted line. Scale bar: 200 µm. N = 25–28. The mRNA expression of anti-apoptotic gene (H) bcl2 and pro-apoptotic genes (I) caspase-3 and (J) bax. n = 24–30. (K) Representative images of ɑ-synuclein and (L) quantified the ɑ-synuclein. A: anterior, P: posterior, L: left, R: right. Scale bar: 10 µm. N = 7–8. Data are presented as the mean ± SD, SD: standard deviation. * p < 0.05, * * p < 0.01, * ** p < 0.001.