FIGURE

Fig. 4

ID
ZDB-FIG-240912-36
Publication
Yang et al., 2024 - Identification of Environmental Compounds That May Trigger Early Female Puberty by Activating Human GnRHR and KISS1R
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Fig. 4

Phosphorylated extracellularly regulated kinase (p-ERK) assay confirmation of gonadotropin-releasing hormone receptor (GnRHR) agonists. A, The Venn diagram shows the p-ERK assay confirmation rate of GnRHR agonist candidates in the HEK293-GnRHR cells. B to F, Concentration-response curves demonstrate that cholinergic agonists, including B, methacholine; C, bethanechol; D, (2-(acryloyloxy)ethyl)trimethylammonium; E, acetylcholine; and F, carbachol, stimulated ERK phosphorylation in HEK293-GnRHR cells but not in wild-type (WT) cells. All values represent the mean ± SD (n = 3 replicates).

Expression Data

Expression Detail
Antibody Labeling
Phenotype Data

Phenotype Detail
Acknowledgments
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