FIGURE

Fig. S1

ID

ZDB-FIG-161208-4

Publication

Topczewska et al., 2016 - The Morphogenesis of Cranial Sutures in Zebrafish

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Fig. S1

RNA detection for genetic markers of osteoblasts in juvenile animals assessed using RNAscope in situ hybridization on paraffin section.

(A-I) Sequential sections (4 μm) of the interfrontal suture collected from juvenile zebrafish at age of 6 wpf. The expression of individual genes is visualized in red, counterstained with haematoxylin for nuclei in purple; black arrows indicate assumed positive expression. The expression of following genes is shown: (A) col2a1a, (B) runx2a, (C) spp1, (D) fgfr1a, (E) fgfr1b, (F) fgfr2, (G) twist2, (H) twist3, (I) foxq1a. The scale bar represents 20 μm.

Expression Data

Genes:	col2a1a fgfr1a fgfr1b fgfr2 foxq1a runx2a spp1 twist2 twist3 (all 9)
Fish:	AB
Anatomical Terms:	inter-frontal joint inter-frontal joint osteoblast mesenchymal cell
Stage:	Days 30-44

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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