PUBLICATION

Single-cell transcriptomic analysis identifies the conversion of zebrafish Etv2-deficient vascular progenitors into skeletal muscle

Authors
Chestnut, B., Casie Chetty, S., Koenig, A.L., Sumanas, S.
ID
ZDB-PUB-200606-5
Date
2020
Source
Nature communications   11: 2796 (Journal)
Registered Authors
Sumanas, Saulius
Keywords
none
Datasets
GEO:GSE142484
MeSH Terms
  • Wnt Signaling Pathway
  • Somites/metabolism
  • Models, Biological
  • Single-Cell Analysis*
  • Embryo, Nonmammalian/metabolism
  • Heat-Shock Response
  • Blood Vessels/cytology*
  • Mutation/genetics
  • Zebrafish/embryology
  • Zebrafish/genetics*
  • Cell Differentiation/genetics
  • Gene Expression Profiling*
  • Zebrafish Proteins/deficiency*
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/metabolism
  • Green Fluorescent Proteins/metabolism
  • Stem Cells/metabolism*
  • Cell Movement
  • Animals, Genetically Modified
  • Animals
  • Transcription, Genetic
  • Fibroblast Growth Factors/metabolism
  • Biomarkers/metabolism
  • Muscle, Skeletal/cytology*
(all 24)
PubMed
32493965 Full text @ Nat. Commun.
Abstract
Cell fate decisions involved in vascular and hematopoietic embryonic development are still poorly understood. An ETS transcription factor Etv2 functions as an evolutionarily conserved master regulator of vasculogenesis. Here we report a single-cell transcriptomic analysis of hematovascular development in wild-type and etv2 mutant zebrafish embryos. Distinct transcriptional signatures of different types of hematopoietic and vascular progenitors are identified using an etv2ci32Gt gene trap line, in which the Gal4 transcriptional activator is integrated into the etv2 gene locus. We observe a cell population with a skeletal muscle signature in etv2-deficient embryos. We demonstrate that multiple etv2ci32Gt; UAS:GFP cells differentiate as skeletal muscle cells instead of contributing to vasculature in etv2-deficient embryos. Wnt and FGF signaling promote the differentiation of these putative multipotent etv2 progenitor cells into skeletal muscle cells. We conclude that etv2 actively represses muscle differentiation in vascular progenitors, thus restricting these cells to a vascular endothelial fate.
Genes / Markers
Figures
Figure Gallery (9 images)
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Expression
Phenotype
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
c264TgTransgenic Insertion
    ci1TgTransgenic Insertion
      ci5TgTransgenic Insertion
        ci32GtTransgenic Insertion
        ci33
          Insertion
          ci46TgTransgenic Insertion
            nkuasgfp1aTgTransgenic Insertion
              pd1TgTransgenic Insertion
                sd2TgTransgenic Insertion
                  w32TgTransgenic Insertion
                    1 - 10 of 15
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                    Human Disease / Model
                    No data available
                    Sequence Targeting Reagents
                    Target Reagent Reagent Type
                    etsrpCRISPR2-etsrpCRISPR
                    etsrpMO1-etsrpMRPHLNO
                    etsrpMO2-etsrpMRPHLNO
                    tal1MO4-tal1MRPHLNO
                    1 - 4 of 4
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                    Fish
                    Antibodies
                    Name Type Antigen Genes Isotypes Host Organism
                    Ab-F310monoclonal
                      IgG1Mouse
                      Ab-S58monoclonal
                        IgAMouse
                        1 - 2 of 2
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                        Orthology
                        No data available
                        Engineered Foreign Genes
                        Marker Marker Type Name
                        CeruleanEFGCerulean
                        DsRedEFGDsRed
                        EGFPEFGEGFP
                        GAL4EFGGAL4
                        GFPEFGGFP
                        mCherryEFGmCherry
                        NTREFGNTR
                        YFPEFGYFP
                        1 - 8 of 8
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                        Mapping
                        No data available