PUBLICATION

Single-cell transcriptomic analysis identifies the conversion of zebrafish Etv2-deficient vascular progenitors into skeletal muscle

Authors
Chestnut, B., Casie Chetty, S., Koenig, A.L., Sumanas, S.
ID
ZDB-PUB-200606-5
Date
2020
Source
Nature communications   11: 2796 (Journal)
Registered Authors
Sumanas, Saulius
Keywords
none
Datasets
GEO:GSE142484
MeSH Terms
  • Heat-Shock Response
  • Zebrafish/embryology
  • Zebrafish/genetics*
  • Blood Vessels/cytology*
  • Fibroblast Growth Factors/metabolism
  • Biomarkers/metabolism
  • Muscle, Skeletal/cytology*
  • Green Fluorescent Proteins/metabolism
  • Animals
  • Cell Movement
  • Models, Biological
  • Mutation/genetics
  • Stem Cells/metabolism*
  • Zebrafish Proteins/deficiency*
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/metabolism
  • Animals, Genetically Modified
  • Wnt Signaling Pathway
  • Transcription, Genetic
  • Somites/metabolism
  • Gene Expression Profiling*
  • Single-Cell Analysis*
  • Cell Differentiation/genetics
  • Embryo, Nonmammalian/metabolism
(all 24)
PubMed
32493965 Full text @ Nat. Commun.
Abstract
Cell fate decisions involved in vascular and hematopoietic embryonic development are still poorly understood. An ETS transcription factor Etv2 functions as an evolutionarily conserved master regulator of vasculogenesis. Here we report a single-cell transcriptomic analysis of hematovascular development in wild-type and etv2 mutant zebrafish embryos. Distinct transcriptional signatures of different types of hematopoietic and vascular progenitors are identified using an etv2ci32Gt gene trap line, in which the Gal4 transcriptional activator is integrated into the etv2 gene locus. We observe a cell population with a skeletal muscle signature in etv2-deficient embryos. We demonstrate that multiple etv2ci32Gt; UAS:GFP cells differentiate as skeletal muscle cells instead of contributing to vasculature in etv2-deficient embryos. Wnt and FGF signaling promote the differentiation of these putative multipotent etv2 progenitor cells into skeletal muscle cells. We conclude that etv2 actively represses muscle differentiation in vascular progenitors, thus restricting these cells to a vascular endothelial fate.
Genes / Markers
Figures
Figure Gallery (9 images)
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Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping
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Citation
Chestnut, B., Casie Chetty, S., Koenig, A.L., Sumanas, S. (2020) Single-cell transcriptomic analysis identifies the conversion of zebrafish Etv2-deficient vascular progenitors into skeletal muscle. Nature communications. 11:2796.