PUBLICATION

Transient inflammatory response mediated by interleukin-1β is required for proper regeneration in zebrafish fin fold

Authors
Hasegawa, T., Hall, C.J., Crosier, P.S., Abe, G., Kawakami, K., Kudo, A., Kawakami, A.
ID
ZDB-PUB-170224-8
Date
2017
Source
eLIFE   6: (Journal)
Registered Authors
Crosier, Phil, Hall, Chris, Kawakami, Atsushi, Kawakami, Koichi, Kudo, Akira
Keywords
developmental biology, stem cells, zebrafish
MeSH Terms
  • Regeneration*
  • Animal Fins/injuries*
  • Animal Fins/physiology*
  • Interleukin-1beta/metabolism*
  • Animals
  • Zebrafish*
  • Inflammation*
(all 7)
PubMed
28229859 Full text @ Elife
Abstract
Cellular responses to injury are recognized to be crucial for complete tissue regeneration, but their underlying processes remain incompletely elucidated. We have previously reported that myeloid-defective zebrafish mutants display apoptosis of regenerative cells during fin fold regeneration. Here, we found that the apoptosis phenotype is induced by the prolonged expression of interleukin 1 beta (il1b). Myeloid cells have been considered to be the principal source of Il1b, but we show that epithelial cells express il1b in response to tissue injury and initiate the inflammatory response, and that its resolution by macrophages is necessary for the survival of regenerative cells. We further show that Il1b also plays an essential role in normal fin fold regeneration by regulating the expression of regeneration-induced genes. Our study reveals that proper levels of Il1b signaling and tissue inflammation, which are tuned by macrophages, play a crucial role in tissue regeneration.
Genes / Markers
Figures
Figure Gallery (12 images) / 2
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Expression
Phenotype
Fish Conditions Stage Phenotype Figure
Df(Chr13:klhdc3,lclat1,npas4l,slc30a10)m39/m39chemical treatment by injection: lipopolysaccharideLong-pec
Df(Chr13:klhdc3,lclat1,npas4l,slc30a10)m39/m39amputation: caudal finLong-pec
Df(Chr13:klhdc3,lclat1,npas4l,slc30a10)m39/m39amputation: caudal finLong-pec
Df(Chr13:klhdc3,lclat1,npas4l,slc30a10)m39/m39amputation: caudal finLong-pec
Df(Chr13:klhdc3,lclat1,npas4l,slc30a10)m39/m39amputation: caudal finLong-pec
Df(Chr13:klhdc3,lclat1,npas4l,slc30a10)m39/m39amputation: caudal finLong-pec
Df(Chr13:klhdc3,lclat1,npas4l,slc30a10)m39/m39amputation: caudal finLong-pec
Df(Chr13:klhdc3,lclat1,npas4l,slc30a10)m39/m39amputation: caudal finLong-pec
Df(Chr13:klhdc3,lclat1,npas4l,slc30a10)m39/m39amputation: caudal finLong-pec
Df(Chr13:klhdc3,lclat1,npas4l,slc30a10)m39/m39amputation: caudal finLong-pec
1 - 10 of 64
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Mutations / Transgenics
Allele Construct Type Affected Genomic Region
m39
    Deficiency
    nkhgn21aEtTransgenic Insertion
      tyt204TgTransgenic Insertion
        tyt205TgTransgenic Insertion
          1 - 4 of 4
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          Human Disease / Model
          No data available
          Sequence Targeting Reagents
          Target Reagent Reagent Type
          csf3rMO1-csf3rMRPHLNO
          csf3rMO4-csf3rMRPHLNO
          il1bMO1-il1bMRPHLNO
          il1bMO3-il1bMRPHLNO
          irf8MO1-irf8MRPHLNO
          spi1bMO1-spi1bMRPHLNO
          1 - 6 of 6
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          Fish
          Antibodies
          No data available
          Orthology
          No data available
          Engineered Foreign Genes
          Marker Marker Type Name
          EGFPEFGEGFP
          mCherryEFGmCherry
          NTREFGNTR
          1 - 3 of 3
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          Mapping
          No data available