Gene
spi1b
- ID
- ZDB-GENE-980526-164
- Name
- Spi-1 proto-oncogene b
- Symbol
- spi1b Nomenclature History
- Previous Names
- Type
- protein_coding_gene
- Location
- Chr: 7 Mapping Details/Browsers
- Description
- Enables sequence-specific DNA binding activity. Involved in macrophage activation involved in immune response and macrophage differentiation. Acts upstream of or within myeloid cell differentiation; negative regulation of myeloid cell differentiation; and regulation of gene expression. Predicted to be active in nucleus. Is expressed in several structures, including hematopoietic cell; hematopoietic system; mesoderm; trunk; and yolk. Human ortholog(s) of this gene implicated in agammaglobulinemia 10. Orthologous to human SPI1 (Spi-1 proto-oncogene).
- Genome Resources
- Note
- None
- Comparative Information
-
- All Expression Data
- 239 figures from 161 publications
- Cross-Species Comparison
- High Throughput Data
- Thisse Expression Data
-
- cb1086 (10 images)
Wild Type Expression Summary
- All Phenotype Data
- 40 figures from 30 publications
- Cross-Species Comparison
- Alliance
Phenotype Summary
Mutations
| Allele | Type | Localization | Consequence | Mutagen | Supplier |
|---|---|---|---|---|---|
| fh509 | Allele with one deletion | Exon 4 | Unknown | CRISPR | |
| hkz4 | Allele with one point mutation | Unknown | Missense | ENU | |
| hkz27 | Allele with one deletion | Unknown | Unknown | TALEN | |
| hkz24 | Allele with one deletion | Unknown | Unknown | TALEN | |
| sa16105 | Allele with one point mutation | Unknown | Splice Site, Premature Stop | ENU | |
| shz4Tg | Transgenic insertion | Unknown | Unknown | DNA and CRISPR | |
| shz5Tg | Transgenic insertion | Unknown | Unknown | DNA and CRISPR |
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| Targeting Reagent | Created Alleles | Citations |
|---|---|---|
| CRISPR1-spi1b | (4) | |
| CRISPR2-spi1b | (2) | |
| CRISPR3-spi1b | (2) | |
| CRISPR4-spi1b | (2) | |
| CRISPR5-spi1b | (2) | |
| CRISPR6-spi1b | (2) | |
| CRISPR7-spi1b | López-Cuevas et al., 2021 | |
| CRISPR8-spi1b | López-Cuevas et al., 2021 | |
| CRISPR9-spi1b | Green et al., 2022 | |
| CRISPR10-spi1b | Yu et al., 2024 |
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Human Disease
| Disease Ontology Term | Multi-Species Data | OMIM Term | OMIM Phenotype ID |
|---|---|---|---|
| agammaglobulinemia 10 | Alliance | Agammaglobulinemia 10, autosomal dominant | 619707 |
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Domain, Family, and Site Summary
Domain Details Per Protein
| Protein | Additional Resources | Length | Ets domain | ETS family | Winged helix DNA-binding domain superfamily | Winged helix-like DNA-binding domain superfamily |
|---|---|---|---|---|---|---|
| UniProtKB:F1R668 | InterPro | 266 | ||||
| UniProtKB:Q78AM8 | InterPro | 296 | ||||
| UniProtKB:A0A8M2BG46 | InterPro | 297 | ||||
| UniProtKB:Q8AW41 | InterPro | 265 |
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Interactions and Pathways
No data available
Plasmids
No data available
| Construct | Regulatory Region | Coding Sequence | Species | Tg Lines | Citations |
|---|---|---|---|---|---|
| Tg(-5.3spi1b:EGFP) |
|
| 1 | (11) | |
| Tg(-9.0spi1b:EGFP) |
|
| 1 | (34) | |
| Tg(spi1b:GAL4,UAS:EGFP) |
|
| 1 | (28) | |
| Tg(-4spi1b:LY-EGFP) |
|
| 1 | (3) | |
| Tg(-9.0spi1b:FLAG-MYC-spi1a) |
|
| 1 | Yu et al., 2017 | |
| Tg(-9.0spi1b:FLAG-MYC-spi1b) |
|
| 1 | Yu et al., 2017 | |
| Tg(hsp70l:MYC-spi1b) |
|
| 1 | (2) | |
| Tg(spi1b:Eos) |
|
| 1 | Green et al., 2022 | |
| Tg(spi1b:GAL4,UAS:LIFEACT-EGFP) |
|
| 1 | Mazaheri et al., 2014 | |
| Tg(spi1b:GAL4,UAS:TagRFP) |
|
| 1 | (16) |
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| Relationship | Marker Type | Marker | Accession Numbers | Citations |
|---|---|---|---|---|
| Contained in | BAC | CH73-314G15 | ZFIN Curated Data | |
| Contained in | BAC | CH211-150G13 | ZFIN Curated Data | |
| Contained in | BAC | XX-184L24 | ||
| Encodes | EST | cb1086 | Thisse et al., 2001 | |
| Encodes | cDNA | MGC:65932 | ZFIN Curated Data | |
| Encodes | cDNA | MGC:85783 | ZFIN Curated Data |
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| Type | Accession # | Sequence | Length (nt/aa) | Analysis |
|---|---|---|---|---|
| RNA | RefSeq:NM_198062 (1) | 2365 nt | ||
| Genomic | GenBank:AL929252 (1) | 173244 nt | ||
| Polypeptide | UniProtKB:A0A8M2BG46 (1) | 297 aa |
- Fallatah, W., Mary, J., Dhinoja, S., Vallabhaneni, S., Jagadeeswaran, P. (2025) Piggyback knockdown screening of unique genes of zebrafish young thrombocytes identifies eight novel genes in thrombopoiesis. Scientific Reports. 15:51805180
- Liu, M., Li, Y., Deng, Z., Zhang, K., Huang, S., Xia, J., Feng, Y., Liang, Y., Sun, C., Liu, X., Li, S., Su, B., Dong, Y., Huang, S. (2025) Mcm5 mutation leads to silencing of Stat1-bcl2 which accelerating apoptosis of immature T lymphocytes with DNA damage. Cell Death & Disease. 16:8484
- Ter Horst, S., Siekierska, A., De Meulemeester, A.S., Cuvry, A., Cools, L., Neyts, J., de Witte, P., Rocha-Pereira, J. (2025) The Dissemination of Rift Valley Fever Virus to the Eye and Sensory Neurons of Zebrafish Larvae Is Stat1-Dependent. Viruses. 17:
- Widziolek, M., Mieszkowska, A., Marcinkowska, M., Salamaga, B., Folkert, J., Rakus, K., Chadzinska, M., Potempa, J., Stafford, G.P., Prajsnar, T.K., Murdoch, C. (2025) Gingipains protect Porphyromonas gingivalis from macrophage-mediated phagocytic clearance. PLoS pathogens. 21:e1012821e1012821
- Xu, S., Hong, J., Dongye, M., Lin, J., Xue, R., Huang, Z., Xu, J., Zhang, Y., Leung, A.Y., Shen, J., Zhang, W., Liu, W. (2025) The synergistic effect of c-Myb hyperactivation and Pu.1 deficiency induces Pelger-Huët anomaly and promotes sAML. Proceedings of the National Academy of Sciences of the United States of America. 122:e2416121122e2416121122
- Ye, Y., Zhao, Z., Mo, W., Liu, W., Wu, L., Li, J., Zhang, W., Huang, Z., Wang, S. (2025) Zebrafish modeling of atypical PML-RARA isoform from acute promyelocytic leukemia patient and its implications for clinical treatment. Annals of Hematology. :
- Belt, A.J., Grant, S., Tombes, R.M., Rothschild, S.C. (2024) Myeloid Targeted Human MLL-ENL and MLL-AF9 Induces cdk9 and bcl2 Expression in Zebrafish Embryos. PLoS Genetics. 20:e1011308e1011308
- Brix, A., Belleri, L., Pezzotta, A., Pettinato, E., Mazzola, M., Zoccolillo, M., Marozzi, A., Monteiro, R., Del Bene, F., Mortellaro, A., Pistocchi, A. (2024) ADA2 regulates inflammation and hematopoietic stem cell emergence via the A2bR pathway in zebrafish. Communications biology. 7:615615
- Doll, L., Welte, K., Skokowa, J., Bajoghli, B. (2024) A JAGN1-associated severe congenital neutropenia zebrafish model revealed an altered G-CSFR signaling and UPR activation. Blood advances. 8(15):4050-4065
- El Omar, R., Abdellaoui, N., Coulibaly, S.T., Fontenille, L., Lanza, F., Gachet, C., Freund, J.N., Negroni, M., Kissa, K., Tavian, M. (2024) Macrophage depletion overcomes human hematopoietic cell engraftment failure in zebrafish embryo. Cell Death & Disease. 15:305305
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