PUBLICATION

An Evolutionarily Conserved Role for Polydom/Svep1 During Lymphatic Vessel Formation

Authors
Kärpanen, T., Padberg, Y., van de Pavert, S.A., Dierkes, C., Morooka, N., Peterson-Maduro, J., van de Hoek, G., Adrian, M., Mochizuki, N., Sekiguchi, K., Kiefer, F., Schulte, D., Schulte-Merker, S.
ID
ZDB-PUB-170210-6
Date
2017
Source
Circulation research   120(8): 1263-1275 (Journal)
Registered Authors
Mochizuki, Naoki, Peterson-Maduroe, Josi, Schulte-Merker, Stefan
Keywords
Lymphangiogenesis, Polydom/Svep1, Zebrafish, lymphatic capillary, mouse model, mouse mutant, valve
MeSH Terms
  • Proteins/genetics
  • Proteins/metabolism*
  • Animals, Genetically Modified
  • Animals
  • Cell Movement
  • Genotype
  • Lymphangiogenesis*
  • Endothelial Cells/metabolism*
  • Endothelial Cells/pathology
  • Phenotype
  • Evolution, Molecular*
  • Time Factors
  • Zebrafish/genetics
  • Zebrafish/metabolism*
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/metabolism*
  • Cell Communication
  • Gene Expression Regulation, Developmental
  • Signal Transduction
  • Lymphatic Vessels/abnormalities
  • Lymphatic Vessels/metabolism*
  • Lymphatic Vessels/physiopathology
  • Endothelium, Lymphatic/abnormalities
  • Endothelium, Lymphatic/metabolism
  • Endothelium, Lymphatic/physiopathology
  • Mesoderm/metabolism
  • Mutation
(all 27)
PubMed
28179432 Full text @ Circ. Res.
Abstract
Lymphatic vessel formation and function constitutes a physiologically and pathophysiologically important process, but its genetic control is not well understood.
Here, we identify the secreted Polydom/Svep1 protein as essential for the formation of the lymphatic vasculature. We analyzed mutants in mice and zebrafish to gain insight into the role of Polydom/Svep1 in the lymphangiogenic process.
Phenotypic analysis of zebrafish polydom/svep1 mutants showed a decrease in venous and lymphovenous sprouting, which leads to an increased number of intersegmental arteries. A reduced number of primordial lymphatic cells populated the horizontal myoseptum region but failed to migrate dorsally or ventrally, resulting in severe reduction of the lymphatic trunk vasculature. Corresponding mutants in the mouse Polydom/Svep1 gene showed normal egression of Prox-1+ cells from the cardinal vein at E10.5, but at E12.5, the tight association between the cardinal vein and lymphatic endothelial cells at the first lymphovenous contact site was abnormal. Furthermore, mesenteric lymphatic structures at E18.5 failed to undergo remodeling events in mutants and lacked lymphatic valves. In both fish and mouse embryos, the expression of the gene suggests a nonendothelial and noncell autonomous mechanism.
Our data identify zebrafish and mouse Polydom/Svep1 as essential extracellular factors for lymphangiogenesis. Expression of the respective genes by mesenchymal cells in intimate proximity with venous and lymphatic endothelial cells is required for sprouting and migratory events in zebrafish and for remodeling events of the lymphatic intraluminal valves in mouse embryos.
Genes / Markers
Figures
Figure Gallery (10 images)
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Expression
Phenotype
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
hu4767
    Point Mutation
    hu5333TgTransgenic Insertion
      hu6123
        Point Mutation
        hu6410
          Point Mutation
          hu6985
            Point Mutation
            hu8885TgTransgenic Insertion
              hu10963
                Small Deletion
                nkuasgfp1aTgTransgenic Insertion
                  s916TgTransgenic Insertion
                    y1TgTransgenic Insertion
                      1 - 10 of 10
                      Show
                      Human Disease / Model
                      No data available
                      Sequence Targeting Reagents
                      Target Reagent Reagent Type
                      ccbe1MO1-ccbe1MRPHLNO
                      itga9TALEN1-itga9TALEN
                      plcg1MO3-plcg1MRPHLNO
                      1 - 3 of 3
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                      Fish
                      Antibodies
                      Name Type Antigen Genes Isotypes Host Organism
                      Ab5-prox1polyclonalIgGRabbit
                      Ab9-mapkmonoclonal
                        IgGRabbit
                        1 - 2 of 2
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                        Orthology
                        No data available
                        Engineered Foreign Genes
                        Marker Marker Type Name
                        EGFPEFGEGFP
                        GAL4FFEFGGAL4FF
                        mCherryEFGmCherry
                        RFPEFGRFP
                        1 - 4 of 4
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                        Mapping
                        No data available