PUBLICATION

RA and FGF Signalling Are Required in the Zebrafish Otic Vesicle to Pattern and Maintain Ventral Otic Identities

Authors
Maier, E.C., Whitfield, T.T.
ID
ZDB-PUB-141205-2
Date
2014
Source
PLoS Genetics   10: e1004858 (Journal)
Registered Authors
Maier, Esther, Whitfield, Tanya T.
Keywords
Embryos, Developmental signaling, Zebrafish, Signal inhibition, Neuroblasts, Ears, Vesicles, Epithelium
MeSH Terms
  • Fibroblast Growth Factors/pharmacology*
  • Organisms, Genetically Modified
  • Signal Transduction/drug effects
  • Signal Transduction/genetics
  • Body Patterning*/drug effects
  • Body Patterning*/genetics
  • Animals
  • Gene Expression Regulation, Developmental/drug effects
  • Ear, Inner/drug effects*
  • Ear, Inner/embryology*
  • Ear, Inner/metabolism
  • Tretinoin/pharmacology*
  • Zebrafish*/embryology
  • Zebrafish*/genetics
  • Embryo, Nonmammalian
(all 15)
PubMed
25473832 Full text @ PLoS Genet.
Abstract
During development of the zebrafish inner ear, regional patterning in the ventral half of the otic vesicle establishes zones of gene expression that correspond to neurogenic, sensory and non-neural cell fates. FGF and Retinoic acid (RA) signalling from surrounding tissues are known to have an early role in otic placode induction and otic axial patterning, but how external signalling cues are translated into intrinsic patterning during otic vesicle (OV) stages is not yet understood. FGF and RA signalling pathway members are expressed in and around the OV, suggesting important roles in later patterning or maintenance events. We have analysed the temporal requirement of FGF and RA signalling for otic development at stages after initial anteroposterior patterning has occurred. We show that high level FGF signalling acts to restrict sensory fates, whereas low levels favour sensory hair cell development; in addition, FGF is both required and sufficient to promote the expression of the non-neural marker otx1b in the OV. RA signalling has opposite roles: it promotes sensory fates, and restricts otx1b expression and the development of non-neural fates. This is surprisingly different from the earlier requirement for RA signalling in specification of non-neural fates via tbx1 expression, and highlights the shift in regulation that takes place between otic placode and vesicle stages in zebrafish. Both FGF and RA signalling are required for the development of the otic neurogenic domain and the generation of otic neuroblasts. In addition, our results indicate that FGF and RA signalling act in a feedback loop in the anterior OV, crucial for pattern refinement.
Genes / Markers
Figures
Figure Gallery (18 images) / 2
Show all Figures
Expression
Phenotype
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
i26
    Point Mutation
    pd18TgTransgenic Insertion
      s356tTgTransgenic Insertion
        sa96
          Point Mutation
          t21142
            Point Mutation
            ti282a
              Point Mutation
              tm208
                Point Mutation
                w2
                  Point Mutation
                  zf115TgTransgenic Insertion
                    1 - 9 of 9
                    Show
                    Human Disease / Model
                    No data available
                    Sequence Targeting Reagents
                    Target Reagent Reagent Type
                    aldh1a3MO1-aldh1a3MRPHLNO
                    1 - 1 of 1
                    Show
                    Fish
                    Antibodies
                    No data available
                    Orthology
                    No data available
                    Engineered Foreign Genes
                    Marker Marker Type Name
                    EGFPEFGEGFP
                    GFPEFGGFP
                    1 - 2 of 2
                    Show
                    Mapping
                    No data available