PUBLICATION

hgf/c-met expression and functional analysis during zebrafish embryogenesis

Authors

Latimer, A.J., and Jessen, J.R.

ID

ZDB-PUB-081203-18

Date

2008

Source

Developmental Dynamics : an official publication of the American Association of Anatomists 237(12): 3904-3915 (Journal)

Registered Authors

Jessen, Jason R., Latimer, Andrew

Keywords

hepatocyte growth factor (HGF), Met, liver development, zebrafish

MeSH Terms

Kidney Tubules/embryology
Kidney Tubules/metabolism
Motor Neurons/metabolism
Animals, Genetically Modified
Proto-Oncogene Proteins c-met/genetics
Proto-Oncogene Proteins c-met/metabolism*
Embryonic Development*/genetics
Female
Zebrafish/embryology*
Zebrafish/genetics
Zebrafish/metabolism*
Gene Expression Regulation, Developmental*
Male
Liver/embryology
Liver/metabolism
Endoderm/embryology
Endoderm/metabolism
Hepatocyte Growth Factor/genetics
Hepatocyte Growth Factor/metabolism*
Nodal Protein/metabolism
Animals
Signal Transduction
Embryo, Nonmammalian/embryology
Embryo, Nonmammalian/metabolism

(all 24)

PubMed

19035351 Full text @ Dev. Dyn.

Abstract

Hepatocyte growth factor (HGF) and its receptor tyrosine kinase Met are linked to several processes underlying vertebrate development and cancer progression. Here, we characterized the expression of zebrafish c-met, hgf1, and hgf2 from cleavage stages through organogenesis and initiated an analysis of Met signaling. We identified c-met as a marker of endoderm and demonstrated that its expression can be activated downstream of Nodal. Injection of c-met mRNA drives expression of the endodermal gene sox17. During gastrulation, hgf1 transcripts are visible in mesendodermal cells along the midline. Later, c-met is expressed in kidney, islet2-positive neurons, and liver. We show that hgf1 is transcribed during gastrulation while hgf1 and hgf2 are detectable in pharyngeal arches and swim bladder. Similar to mouse, knockdown of zebrafish Met reduces liver size. Our results suggest a role for Met during endoderm specification and indicate that mechanisms of liver development are conserved between mammals and bony fish.

Genes / Markers

Figures

Show all Figures

Expression

Gene	Fish	Conditions	Stage	Anatomy	Assay	Figure
cp	AB	control	Long-pec	liver	ISH	Fig. 4
cp	AB + MO1-met	standard conditions	Long-pec	liver	ISH	Fig. 4
foxa3	AB	standard conditions	14-19 somites	endodermal cell	ISH	Fig. 2
foxa3	AB	standard conditions	Protruding-mouth	gut	ISH	Fig. 4
foxa3	AB	standard conditions	Protruding-mouth	liver	ISH	Fig. 4
foxa3	AB	control	Long-pec	liver	ISH	Fig. 4
foxa3	AB + MO1-met	standard conditions	14-19 somites	endodermal cell	ISH	Fig. 2
foxa3	AB + MO1-met	standard conditions	Protruding-mouth	gut	ISH	Fig. 4
foxa3	AB + MO1-met	standard conditions	Protruding-mouth	liver	ISH	Fig. 4
foxa3	AB + MO1-met	standard conditions	Long-pec	liver	ISH	Fig. 4

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Phenotype

Fish	Conditions	Stage	Phenotype	Figure
AB + MO1-met	standard conditions	14-19 somites	endodermal cell amount, normal	Fig. 2
AB + MO1-met	standard conditions	14-19 somites	endodermal cell spatial pattern, normal	Fig. 2
AB + MO1-met	standard conditions	Prim-5	liver primordium morphology, normal	Fig. 4
AB + MO1-met	standard conditions	Prim-5	pronephros development growth rate, normal	Fig. 4
AB + MO1-met	standard conditions	Long-pec	pectoral fin morphology, abnormal	Fig. 2
AB + MO1-met	standard conditions	Protruding-mouth	liver decreased size, abnormal	Fig. 4
AB + MO1-met	standard conditions	Protruding-mouth	liver development disrupted, abnormal	Fig. 4
AB + MO1-met	standard conditions	Protruding-mouth	whole organism curved ventral, abnormal	Fig. 4
AB + MO1-met	standard conditions	Long-pec	liver decreased size, abnormal	Fig. 4
AB + MO1-met	standard conditions	Long-pec	liver development disrupted, abnormal	Fig. 4

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Mutations / Transgenics

Allele	Construct	Type	Affected Genomic Region
tz257		Point Mutation	tdgf1

Human Disease / Model

Sequence Targeting Reagents

Target	Reagent	Reagent Type
met	MO1-met	MRPHLNO
met	MO4-met	MRPHLNO
met	MO5-met	MRPHLNO

Fish

Fish
AB
AB + MO1-met
AB + MO4-met
AB + MO5-met
tdgf1^tz257/+
tdgf1^tz257/tz257

Antibodies

Orthology

Engineered Foreign Genes

Mapping

Latimer et al., 2008 ZDB-PUB-081203-18 PMID:19035351

hgf/c-met expression and functional analysis during zebrafish embryogenesis

Latimer et al., 2008

ZDB-PUB-081203-18
PMID:19035351