PUBLICATION

Pancreas development in zebrafish: early dispersed appearance of endocrine hormone expressing cells and their convergence to form the definitive islet

Authors
Biemar, F., Argenton, F., Schmidtke, R., Epperlein, S., Peers, B., and Driever, W.
ID
ZDB-PUB-010205-4
Date
2001
Source
Developmental Biology   230(2): 189-203 (Journal)
Registered Authors
Argenton, Francesco, Biemar, Frédéric, Driever, Wolfgang, Epperlein, Simone, Peers, Bernard
Keywords
zebrafish; pancreas; endoderm; insulin
MeSH Terms
  • Mutation
  • Zebrafish/embryology*
  • Zebrafish/genetics*
  • Species Specificity
  • Morphogenesis
  • Transcription Factors/genetics
  • Transcription Factors/metabolism
  • DNA, Complementary
  • Molecular Sequence Data
  • Embryo, Nonmammalian/physiology
  • Mesoderm/physiology
  • Insulin/genetics
  • Gene Expression Regulation, Developmental*
  • Base Sequence
  • Pancreas/cytology
  • Pancreas/embryology*
  • Islets of Langerhans/cytology
  • Islets of Langerhans/embryology*
  • Trypsin/genetics*
  • Animals
  • Gene Expression Regulation, Enzymologic
  • Amino Acid Sequence
(all 22)
PubMed
11161572 Full text @ Dev. Biol.
Abstract
To begin to understand pancreas development and the control of endocrine lineage formation in zebrafish, we have examined the expression pattern of several genes shown to act in vertebrate pancreatic development: pdx-1, insulin (W. M. Milewski et al., 1998, Endocrinology 139, 1440-1449), glucagon, somatostatin (F. Argenton et al., 1999, Mech. Dev. 87, 217-221), islet-1 (Korzh et al., 1993, Development 118, 417-425), nkx2.2 (Barth and Wilson, 1995, Development 121, 1755-1768), and pax6.2 (Nornes et al., 1998, Mech. Dev. 77, 185-196). To determine the spatial relationship between the exocrine and the endocrine compartments, we have cloned the zebrafish trypsin gene, a digestive enzyme expressed in differentiated pancreatic exocrine cells. We found expression of all these genes in the developing pancreas throughout organogenesis. Endocrine cells first appear in a scattered fashion in two bilateral rows close to the midline during mid-somitogenesis and converge during late-somitogenesis to form a single islet dorsal to the nascent duodenum. We have examined development of the endocrine lineage in a number of previously described zebrafish mutations. Deletion of chordamesoderm in floating head (Xnot homolog) mutants reduces islet formation to small remnants, but does not delete the pancreas, indicating that notochord is involved in proper pancreas development, but not required for differentiation of pancreatic cell fates. In the absence of knypek gene function, which is involved in convergence movements, the bilateral endocrine primordia do not merge. Presence of trunk paraxial mesoderm also appears to be instrumental for convergence since the bilateral endocrine primordia do not merge in spadetail mutants. We discuss our findings on zebrafish pancreatogenesis in the light of evolution of the pancreas in chordates.
Genes / Markers
Figures
Figure Gallery (6 images)
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Expression
Phenotype
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
b104
    Indel
    b160
      Indel
      m93
        Point Mutation
        m134
          Point Mutation
          m768
            Point Mutation
            m818
              Point Mutation
              tk241
                Point Mutation
                tz216
                  Point Mutation
                  1 - 8 of 8
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                  Human Disease / Model
                  No data available
                  Sequence Targeting Reagents
                  No data available
                  Fish
                  Antibodies
                  Orthology
                  Gene Orthology
                  pdx1
                  1 - 1 of 1
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                  Engineered Foreign Genes
                  No data available
                  Mapping
                  No data available