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Fig. 3 Bone morphogenetic protein (BMP) Pathway target genes are expressed in crestin+ and hand2+ progenitors. Schematic of regions of interest in the zebrafish embryo (A). Whole-mount hybridization chain reaction (HCR) analysis of msx1b and crestin co-expression at 24 hpf (C–E) and 36 hpf (F–H), or id2a and hand2 co-expression at 48 hpf (I–K) and 72 hpf (L–N). White arrows denote regions of co-expression along the dorsal aspect (C, D, E), or in the gut tube (F–N). The dot plot demonstrates the expression of select cell identity and BMP pathway categorical identifiers with intracellular effectors across major developmental times (O). Anterior oriented to the left and dorsal to the top, in these images. The scale bar depicts 50 microns in C, F, I, and L. P.o.D, Post-otic Domain; OV, Otic vesicle, ≥3 experiments with three biological replicates.