PUBLICATION
BMP signaling pathway member expression is enriched in enteric neural progenitors and required for zebrafish enteric nervous system development
- Authors
- Moore, J.A., Moreno-Campos, R., Noah, A.S., Singleton, E.W., Uribe, R.A.
- ID
- ZDB-PUB-240923-2
- Date
- 2024
- Source
- Developmental Dynamics : an official publication of the American Association of Anatomists : (Journal)
- Registered Authors
- Singleton, Eileen, Uribe, Rosa
- Keywords
- BMP, bmp5, enteric nervous system, enteric nervous system development, enteric neural crest, zebrafish
- MeSH Terms
-
- Zebrafish Proteins*/genetics
- Zebrafish Proteins*/metabolism
- Signal Transduction*
- Enteric Nervous System*/cytology
- Enteric Nervous System*/embryology
- Enteric Nervous System*/metabolism
- Neural Crest/cytology
- Neural Crest/metabolism
- Zebrafish*
- Neural Stem Cells/cytology
- Neural Stem Cells/metabolism
- Animals
- Cell Differentiation
- Gene Expression Regulation, Developmental
- Bone Morphogenetic Proteins*/genetics
- Bone Morphogenetic Proteins*/metabolism
- PubMed
- 39297504 Full text @ Dev. Dyn.
Citation
Moore, J.A., Moreno-Campos, R., Noah, A.S., Singleton, E.W., Uribe, R.A. (2024) BMP signaling pathway member expression is enriched in enteric neural progenitors and required for zebrafish enteric nervous system development. Developmental Dynamics : an official publication of the American Association of Anatomists. :.
Abstract
Background The vertebrate enteric nervous system (ENS) consists of a series of interconnected ganglia within the gastrointestinal (GI) tract, formed during development following migration of enteric neural crest cells (ENCCs) into the primitive gut tube. Much work has been done to unravel the complex nature of extrinsic and intrinsic factors that regulate processes that direct migration, proliferation, and differentiation of ENCCs. However, ENS development is a complex process, and we still have much to learn regarding the signaling factors that regulate ENCC development.
Results Here in zebrafish, through transcriptomic, in situ transcript expression, immunohistochemical analysis, and chemical attenuation, we identified a time-dependent role for bone morphogenetic protein (BMP) in the maintenance of Phox2bb+ enteric progenitor numbers and/or time of differentiation of the progenitor pool. In support of our in silico transcriptomic analysis, we identified expression of a novel ENS ligand-encoding transcript, bmp5, within developmental regions of ENCCs. Through generation of a novel mutant bmp5wmr2 and bmp5 crispants, we identified a functional role for BMP5 in proper GI tract colonization, whereby phox2bb+ enteric progenitor numbers were reduced.
Conclusion Altogether, this work identified time-dependent roles for BMP signaling and a novel extrinsic factor, BMP5, that is necessary for vertebrate ENS formation.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping