FIGURE

Fig. 3

ID

ZDB-FIG-250716-3

Publication

Dhillon-Richardson et al., 2025 - Reactivation of an embryonic cardiac neural crest transcriptional profile during zebrafish heart regeneration

Other Figures

All Figure Page

Back to All Figure Page

Fig. 3

Loss of egr1 leads to a downregulation of CdNC genes and suggests an important role as core CdNC subcircuit transcription factor. HCR staining for ets1 and sox9a in 16ss zebrafish embryos injected with negative control gRNA (n = 11) (A and A”) and egr1 gRNA (n = 9) (B and B”). HCR staining for tfap2a and sox10 in 16ss zebrafish embryos injected with negative control gRNA (n = 9) (C and C″) and egr1 gRNA (n = 14) (D and D”). (E–H) Quantification of CTCF in negative control and egr1 gRNA injected embryos (unpaired Student’s t test P < 0.05). (I) Migratory CdNC subcircuit displaying proposed interactions between shown transcription factors and downstream enhancer regions. (Scale bar, 50 μm.)

Expression Data

Genes:	ets1 sox9a sox10 tfap2a
Fish:	AB AB + CRISPR14-egr1
Knockdown Reagent:	CRISPR14-egr1
Anatomical Term:	cardiac neural crest
Stage:	14-19 somites

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	AB + CRISPR14-egr1
Knockdown Reagent:	CRISPR14-egr1
Observed In:	cardiac neural crest
Stage:	14-19 somites

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Proc. Natl. Acad. Sci. USA