Fig. 6

Additional fshβ depletion in cgα-deficient zebrafish skews gonadal sex differentiation towards males A: Western blot analysis of Fshβ and Lhβ proteins in head tissue containing pituitary from control and cgα-deficient fish at 14, 28, and 42 dpf. B: Fshβ protein levels were significantly increased in cgα-deficient fish at 28 dpf, but not at 42 dpf. C: Lhβ protein levels remained unchanged in cgα-deficient fish compared to controls at 42 dpf. D–G: Histological analysis of control and cgα−/−;fshβ−/− fish at 4 mpf. H: Sex ratio of control, cgα−/−, and cgα−/−;fshβ−/− fish at 4 mpf. I: Transcription of male pathway-related genes showed no significant changes in cgα-deficient male fish compared to controls at 4 mpf, including amh, sox9a, dmrt1, and cyp17a1. J: Transcription of female pathway-related genes showed no significant changes in cgα-deficient female fish compared to controls at 4 mpf, including foxl2a and cyp19a1a. K: Transcription of amh was significantly up-regulated in cgα−/−;fshβ−/− male fish compared to controls at 4 mpf, although no significant alteration in transcription of sox9a, dmrt1, and cyp17a1 was observed. L: Transcription of foxl2a and cyp19a1a showed no significant changes in cgα−/−;fshβ−/− female fish compared to controls at 4 mpf. dpf, days post-fertilization; SG, spermatogonia; SC, spermatocytes; SZ, spermatozoa; PG, primary growth follicle; PV, previtellogenic follicle; EV, early vitellogenic follicle; MV, middle vitellogenic follicle; FG, full-grown follicle. ns: Not significant; *: P<0.05; **: P<0.01.