FIGURE

Fig. 2

ID

ZDB-FIG-250425-2

Publication

Yang et al., 2025 - Optimization of genome editing by CRISPR ribonucleoprotein for high efficiency of germline transmission of Sox9 in zebrafish

Other Figures

All Figure Page

Back to All Figure Page

Fig. 2

Typing of genome editing and germline transmission. A. Breeding scheme. Founders (F0) are crossed with wild-type animals to determine the transmission of the edited genes in the F1 generation. B. Statistical analysis of the types of edited genes in the F1 generation in 3 families of sox9a (f1 to f3) and 4 families of akirin2 (f1 to f4) after PCR cloning of the edited genes and sequencing. Different shades of blue indicate different types of editing, and the wild-type (WT) is shown in orangesingle bondyellow. n = 30 individuals/family. C, D. Significance testing of the germline transmission ratio of edited genes from the founder to the F1 generation by two-way ANOVA in the sox9a group (C) and the akirin2 group (D). The dotted red lines show the Mendel values. P values are indicated for each group. n = 30 individuals/family.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image.

Reprinted from New biotechnology, , Yang, K., Cai, L., Zhao, Y., Cheng, H., Zhou, R., Optimization of genome editing by CRISPR ribonucleoprotein for high efficiency of germline transmission of Sox9 in zebrafish, , Copyright (2025) with permission from Elsevier. Full text @ N. Biotechnol.