Fig. 2

Fig. 2 Typing of genome editing and germline transmission. A. Breeding scheme. Founders (F0) are crossed with wild-type animals to determine the transmission of the edited genes in the F1 generation. B. Statistical analysis of the types of edited genes in the F1 generation in 3 families of sox9a (f1 to f3) and 4 families of akirin2 (f1 to f4) after PCR cloning of the edited genes and sequencing. Different shades of blue indicate different types of editing, and the wild-type (WT) is shown in orangesingle bondyellow. n = 30 individuals/family. C, D. Significance testing of the germline transmission ratio of edited genes from the founder to the F1 generation by two-way ANOVA in the sox9a group (C) and the akirin2 group (D). The dotted red lines show the Mendel values. P values are indicated for each group. n = 30 individuals/family.