FIGURE

Fig. 7

ID

ZDB-FIG-250409-47

Publication

Moore et al., 2024 - BMP signaling pathway member expression is enriched in enteric neural progenitors and required for zebrafish enteric nervous system development

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Fig. 7

CRISPR mediated mutation in bmp5 reduces − 8.3phox2bb:Kaede+ enteric progenitors. Schematic of bmp5 and CRISPR target regions, red arrows denote the location of the 2 sgRNAs (A). Agarose gel analysis of bmp5 target region amplicon via PCR and T7 endonuclease 1 activity (B). Fraction of ENS phenotypic distributions for bmp5wmr2/+, F2 bmp5wmr2/+, F2 bmp5wmr2/ wmr2, and bmp5 crispant larvae (C). Kaede+ cell reduction in bmp5 F1s at 120 hpf, and F2s and crispants at 96 hpf (E–I), when compared to bmp5+/+ ENS (D, F). The respective sequences around the targeted region of interest, with bmp5+/+ and F1 bmp5wmr2/+ containing a 16-bp insertion (D, E right). Number of Kaede+ cells of the different bmp5 F1s, F2s and crispants in the gut (J, K). ≥3 experiments with three biological replicates. T test p-value *, .0117; ANOVA p-value ****, <0.0001; ***, 0.0008; *, <.01. Scale bar depicts 50 microns.

Expression Data

Gene:	Kaede
Fish:	em2Tg em2Tg + CRISPR4-bmp5 bmp5^wmr2/+; em2Tg bmp5^wmr2/wmr2; em2Tg
Knockdown Reagent:	CRISPR4-bmp5
Anatomical Term:	enteric neuron
Stage Range:	Day 4 to Day 5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	em2Tg + CRISPR4-bmp5 bmp5^wmr2/+; em2Tg bmp5^wmr2/wmr2; em2Tg
Knockdown Reagent:	CRISPR4-bmp5
Observed In:	enteric nervous system enteric nervous system development enteric neuron
Stage Range:	Day 4 to Day 5

Phenotype Detail

Acknowledgments

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