FIGURE

Fig. 5

ID

ZDB-FIG-250409-45

Publication

Moore et al., 2024 - BMP signaling pathway member expression is enriched in enteric neural progenitors and required for zebrafish enteric nervous system development

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Fig. 5

K02288 mediated attenuation of bone morphogenetic protein (BMP) signaling reduced enteric progenitor number in gut tube in a time-dependent manner. Schematic of the region of interest to be imaged for counting enteric neurons in the zebrafish embryo (A). Schematic of treatment paradigm with 8 μM K02288 (B). Whole-mount confocal imaging of the combinatorial expression of Kaede+ and Elavl3/4+ cells after 24–120 hpf DMSO control (C–E), or 24–120hpf (F–H), 36–120 hpf (I–K) 8 μM K02288 treatments. Counts of Kaede+ cells, Elavl3/4+ cells, or percentage of co-positive cells over Kaede+ cells using Imaris image analysis software (L). Measure of midgut thickness, determined using raw confocal images and conversion of pixel number to microns in ImageJ. Anterior oriented to the left and dorsal to the top, in these images. Scale bar in C, F, and I denotes 50 microns. ≥ 3 experiments with three biological replicates. * Denotes p-value < .05.

Expression Data

Gene:	Kaede
Antibodies:	Ab1-elavl Ab3-phox2bb Ab13-smad
Fish:	AB em2Tg
Condition:	chemical treatment by environment: bone morphogenetic protein receptor antagonist
Anatomical Terms:	enteric neuron gut
Stage:	Day 5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	AB WT em2Tg
Condition:	chemical treatment by environment: bone morphogenetic protein receptor antagonist
Observed In:	enteric nervous system enteric nervous system neuron differentiation enteric nervous system development enteric neuron gut mid intestine
Stage:	Day 5

Phenotype Detail

Acknowledgments

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