FIGURE

Fig. 8

ID

ZDB-FIG-250408-17

Publication

Lv et al., 2025 - Angiogenic factor AGGF1 is a general splicing factor regulating angiogenesis and vascular development by alternative splicing of SRSF6

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Fig. 8

AGGF1 and SRSF6-FL promote capillary angiogenesis of EA.hy926 cells and ISV vascular development in zebrafish. (A) Capillary tube formation for angiogenesis for EA.hy926 cells transfected with different siRNA (KD) or mammalian expression plasmid DNA (OE) or combination (n = 5 experiments). ***p < .001, ****p < .0001 vs. Control; ns, no significance. (B) Sanger sequencing panels showing mosaic knockout (KO) of srsf6a and srsf6b in zebrafish at F0 using the CRISPR/Cas9 system. (C, D) Whole-mount in situ hybridization with markers genes kdrl and cdh5 for intersegmental vessels (ISVs) in 26 hpf zebrafish embryos. (C) KO of srsf6a and srsf6b impaired the development of ISVs. (D) Overexpression of the full-length SRSF6-FL mRNA (mCherrry RNA as negative control) enhanced the development of ISVs in zebrafish. Scale bar, 200 μm; n = 16 to 19 embryos per group.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	AB + CRISPR1-srsf6a + CRISPR1-srsf6b
Knockdown Reagents:	CRISPR1-srsf6a CRISPR1-srsf6b
Observed In:	blood vessel development intersegmental vessel
Stage:	Prim-5

Phenotype Detail

Acknowledgments

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