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Fig. 8 AGGF1 and SRSF6-FL promote capillary angiogenesis of EA.hy926 cells and ISV vascular development in zebrafish. (A) Capillary tube formation for angiogenesis for EA.hy926 cells transfected with different siRNA (KD) or mammalian expression plasmid DNA (OE) or combination (n = 5 experiments). ***p < .001, ****p < .0001 vs. Control; ns, no significance. (B) Sanger sequencing panels showing mosaic knockout (KO) of srsf6a and srsf6b in zebrafish at F0 using the CRISPR/Cas9 system. (C, D) Whole-mount in situ hybridization with markers genes kdrl and cdh5 for intersegmental vessels (ISVs) in 26 hpf zebrafish embryos. (C) KO of srsf6a and srsf6b impaired the development of ISVs. (D) Overexpression of the full-length SRSF6-FL mRNA (mCherrry RNA as negative control) enhanced the development of ISVs in zebrafish. Scale bar, 200 μm; n = 16 to 19 embryos per group.