Unchallenged as well as M. marinum-infected pycardtpu4/tpu4 mutants have normal distribution of blood cell populations. Adult zebrafish were either mock injected (PBS) or infected with a low dose (experiment 1: mean, 17 CFU; range, 12-28 CFU; experiment 2: mean, 19 CFU; range 16-21 CFU) of M. marinum (Mmar). The whole-kidney marrow was analysed at 4 wpi using flow cytometry. Results have been pooled from two experiments (n=3-5 in each group, per experiment). Each datapoint represents an individual fish. The line indicates the median. See Fig. S4 for the individual experiments, including exact sample size, bacterial dose and the gating strategy. Both sexes were included in the experiment in approximately equal numbers.
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