FIGURE

Figure 7.

ID

ZDB-FIG-250122-33

Publication

Guo et al., 2025 - Cre-Lox miRNA-delivery technology optimized for inducible microRNA and gene-silencing studies in zebrafish

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Figure 7.

Cell-specific miR-delivery/RNAi in zebrafish does not require transposase. We tested the ability of the presented miR-delivery system to enable rapid cell-specific experiments. The approach is similar to the method conducted in Figures 2 and 3 but aimed at evaluating the effect of different injection mix. (A) Schematic example of the expression obtained following the injection of a motoneuron-specific miR-delivery construct (83_HB9_mKate_smn4141) within the cell of one-cell stage tg(MN:GFP) animals. Arrowhead indicating mKate positive motoneurons with positive transgene expression. (B) Titration/Toxicity test of Tol2-construct injections (83_HB9_mKate_smn4141) for triggering cell-specific expression. (C) Number (percentage) of animals presenting at least 1× mKate positive motoneuron. (D) Average number of mKate-positive CaP motoneurons per positive animal. Eighty embryos per condition have been analyzed (means ± SEM). These results suggest that a dose of 50 pg without transposase may be ideal for setting up cell-specific approaches and studies.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Nucleic Acids Res.