ZFIN Image: Guo et al., 2025, Figure 7.

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Figure Caption

Figure 7.

Cell-specific miR-delivery/RNAi in zebrafish does not require transposase. We tested the ability of the presented miR-delivery system to enable rapid cell-specific experiments. The approach is similar to the method conducted in Figures 2 and 3 but aimed at evaluating the effect of different injection mix. (A) Schematic example of the expression obtained following the injection of a motoneuron-specific miR-delivery construct (83_HB9_mKate_smn4141) within the cell of one-cell stage tg(MN:GFP) animals. Arrowhead indicating mKate positive motoneurons with positive transgene expression. (B) Titration/Toxicity test of Tol2-construct injections (83_HB9_mKate_smn4141) for triggering cell-specific expression. (C) Number (percentage) of animals presenting at least 1× mKate positive motoneuron. (D) Average number of mKate-positive CaP motoneurons per positive animal. Eighty embryos per condition have been analyzed (means ± SEM). These results suggest that a dose of 50 pg without transposase may be ideal for setting up cell-specific approaches and studies.

Acknowledgments

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