Fig. 3

OCLN-derived EL2 peptide inhibits human monocyte transmigration in vivo. (A) Representative scheme of the experimental design associated with the zebrafish model. Human primary monocytes were labeled with CellTrace, and injected into the duct of Cuvier of Tg(fli1a:eGFP-CAAX) zebrafish embryos (GFP-labeled endothelial cells). (B) Zebrafish imaging was done at 6–8 h post injection by spinning-disk confocal microscopy. Representative z-projection of confocal images of monocytes from patient X arrested in the vasculature at the tail of a zebrafish embryo at 6 h post injection Scale bar: 100 µm. (C) Representative images and orthoslice of monocytes at 6 hpi. Scale bar: 50 µm. (D) Violin plots of the ratio of extravasated monocytes from four donors (merged and seperated) at 6–8 h post injection. Each dot represents the ratio calculated from all the monocytes tracked within a fish. The data were obtained from n = 4 individual experiments using four different monocyte donors with nDMSO = 21, nEL1 = 31, nEL2 = 25 in total (nDMSO = 7, nEL1 = 10, nEL2 = 6 for donor W; nDMSO = 6, nEL1 = 7, nEL2 = 4 for donor X; nDMSO = 6, nEL1 = 8, nEL2 = 8 for donor Y; nDMSO = 2, nEL1 = 6, nEL2 = 7 for donor Z). P value < 0.05 (*), and <0.0001 (***). Data information: In (D), data are presented as violin plots showing min/max values and quartiles (dotted lines). Statistical difference of non-Gaussian datasets was analyzed using Kruskal–Wallis test with Dunn’s post hoc test p value < 0.05 (*), < 0.001 (***) or non-significant (ns). Source data are available online for this figure.