FIGURE

FIGURE 3

ID

ZDB-FIG-230821-9

Publication

Tromp et al., 2023 - Optimising the zebrafish Cre/Lox toolbox. Codon improved iCre, new gateway tools, Cre protein and guidelines

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FIGURE 3

iCre-mRNA injections successfully trigger Lox-recombination but still with some visible partial conversions. (A–C), Control injections demonstrating no spontaneous Lox-recombination in absence of iCre. (D–L), Injection of 50pg–300 pg of iCre mRNA at one-cell stage is enough to promote Lox-recombination. However, partial conversion is still observed with the line studied. At 300 pg, most animal (81%) were however successfully fully converted with no visible blue fluorescence. Scale bar 100 µm.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Front. Physiol.