FIGURE

FIGURE 4

ID

ZDB-FIG-230821-10

Publication

Tromp et al., 2023 - Optimising the zebrafish Cre/Lox toolbox. Codon improved iCre, new gateway tools, Cre protein and guidelines

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FIGURE 4

Female iCre-driver tg(UBI:iCre) triggers 100% recombination of Lox-Responder transgenes. (A–C), When our Lox-Responder tg(UBI:LoxSENSOR) is crossed against a wildtype control, no Lox-recombination is observed and all analysed animals presented with homogeneous BFP/blue fluorescence. (D–F), When Females tg(UBI:iCre) are crossed against Males tg(UBI:LoxSENSOR), 100% all clutches observed presented with homogeneous and fully converted Lox-transgenes; i.e., all animals presented Red/mKate fluorescence without any visible Blue/BFP fluorescence, evidencing complete Flox-recombination in all animals observed. (G–I), When Males tg(UBI:iCre) are crossed against Females tg(UBI:LoxSENSOR), >70% of animals presented with homogeneous full Lox-recombination (Figure 2E), but a few animals remain only partially floxed, evidenced by the presence of around 30% of animals presenting both Red/mKate and Blue/BFP fluorescence. Scale bar 100 µm.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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