FIGURE

FIGURE 5

ID

ZDB-FIG-230821-11

Publication

Tromp et al., 2023 - Optimising the zebrafish Cre/Lox toolbox. Codon improved iCre, new gateway tools, Cre protein and guidelines

Other Figures

All Figure Page

Back to All Figure Page

FIGURE 5

Cre protein injections successfully trigger Lox-recombination. (A–C), Control injections demonstrating no spontaneous Lox-recombination in the absence of Cre protein. (D–R), Injection of 10 pg to 10 ng of cell-permeant Cre protein at one-cell stage. No significant Lox-recombination is observed from 10pg to 100 pg. However, robust conversions are observed from 0.5 ng to 10ng, with 100% of the animal injected with 10 ng presenting total conversion of the Lox-transgenes (i.e., only Red/mKate fluorescence was detected). Note that protein injections may also be an attractive approach for local Lox-recombination at a later stage of zebrafish development. Scale bar 100 µm.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Front. Physiol.