FIGURE

Fig. 4

ID

ZDB-FIG-200101-48

Publication

Li et al., 2019 - SOX19b regulates the premature neuronal differentiation of neural stem cells through EZH2-mediated histone methylation in neural tube development of zebrafish

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Fig. 4

NSCs in embryos injected with sox19b MO were prematurely differentiated. a Quantitative RT-PCR analysis for Ngn1, ascl1a, HuC, lslet1, and her3. Compared with the control group, the mRNA levels of Ngn1, ascl1a, HuC, and lslet1 in Sox19b MO-injection embryos increased significantly, but the mRNA level of her3 decreased significantly. b The expression levels of Ngn1, HuC, and lslet1 were detected by whole-mount in situ hybridization. Bar graphs show the percentage of normal embryos in each group. Data represent the mean of at least three independent experiments ± SD. *P < 0.05, **P < 0.01 versus control. c Quantitative RT-PCR analysis is shown for p21, p27, and p57. Knockdown of Sox19b caused a significant increase in the mRNA levels of cyclin-dependent kinase inhibitor. Data represent the mean of at least three independent experiments ± SD. *P < 0.05 versus control. d Control and Sox19b MO-injected embryos were analysed by immunofluorescence staining of PHH3 (a marker of mitosis, red). Data represent the mean of at least three independent experiments ± SD. *P < 0.05 versus control

Expression Data

Genes:	ascl1a cdkn1a cdkn1bb cdkn1ca elavl3 her3 isl1a neurog1 (all 8)
Antibody:	Ab36-h3
Fish:	WT WT + MO6-sox19b WT + MO7-sox19b
Knockdown Reagents:	MO6-sox19b MO7-sox19b
Anatomical Terms:	neural plate neural plate cell whole organism
Stage:	Bud

Expression Detail

Gene	Fish	Conditions	Stage	Anatomy	Assay
ascl1a	WT	standard conditions	Bud to 1-4 somites	whole organism	RTPCR
ascl1a	WT + MO6-sox19b	standard conditions	Bud to 1-4 somites	whole organism	RTPCR
ascl1a	WT + MO7-sox19b	standard conditions	Bud to 1-4 somites	whole organism	RTPCR
cdkn1a	WT	standard conditions	Bud to 1-4 somites	whole organism	RTPCR
cdkn1a	WT + MO6-sox19b	standard conditions	Bud to 1-4 somites	whole organism	RTPCR
cdkn1a	WT + MO7-sox19b	standard conditions	Bud to 1-4 somites	whole organism	RTPCR
cdkn1bb	WT	standard conditions	Bud to 1-4 somites	whole organism	RTPCR
cdkn1bb	WT + MO6-sox19b	standard conditions	Bud to 1-4 somites	whole organism	RTPCR
cdkn1bb	WT + MO7-sox19b	standard conditions	Bud to 1-4 somites	whole organism	RTPCR
cdkn1ca	WT	standard conditions	Bud to 1-4 somites	whole organism	RTPCR

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Antibody Labeling

Phenotype Data

Fish:	WT + MO6-sox19b WT + MO7-sox19b
Knockdown Reagents:	MO6-sox19b MO7-sox19b
Observed In:	neural plate neural plate cell whole organism
Stage Range:	Bud to 1-4 somites

Phenotype Detail

Acknowledgments

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