FIGURE

Fig. 7

ID

ZDB-FIG-170606-33

Publication

Naylor et al., 2017 - Wnt8a expands the pool of embryonic kidney progenitors in zebrafish

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Fig. 7

Effect of wnt8a knockdown on inter-nuclear distance. A) Lateral views of 24 hpf embryos stained for slc4a4 (red) and slc12a3 (purple). Black arrows identify the anterior end of the slc12a3⁺ DL segment. B) Panels show lateral views of the pronephric tubule (proximal left, distal right) stained for Hnf1b to identify the nucleus of renal epithelial cells in response to wnt8a knockdown and/or HUA treatment. Histogram shows the average inter-nuclear distances of cells in the proximal region of the pronephros for each treatment.

Expression Data

Genes:	slc4a4a slc12a3
Antibody:	Ab1-hnf1b
Fish:	TU TU + MO1-wnt8a + MO2-wnt8a
Condition:	chemical treatment by environment: aphidicolin
Knockdown Reagents:	MO1-wnt8a MO2-wnt8a
Anatomical Terms:	pronephric tubule distal region pronephric tubule proximal region
Stage:	Prim-5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	TU TU + MO1-wnt8a + MO2-wnt8a
Condition:	chemical treatment by environment: aphidicolin
Knockdown Reagents:	MO1-wnt8a MO2-wnt8a
Observed In:	pronephric tubule cell pronephric tubule distal region
Stage:	Prim-5

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 425(2), Naylor, R.W., Han, H.I., Hukriede, N.A., Davidson, A.J., Wnt8a expands the pool of embryonic kidney progenitors in zebrafish, 130-141, Copyright (2017) with permission from Elsevier. Full text @ Dev. Biol.