PUBLICATION

Mutations affecting cell fates and cellular rearrangements during gastrulation in zebrafish

Authors
Solnica-Krezel, L., Stemple, D.L., Mountcastle-Shah, E., Rangini, Z., Neuhauss, S.C., Malicki, J., Schier, A.F., Stainier, D.Y., Zwartkruis, F., Abdelilah, S., and Driever, W.
ID
ZDB-PUB-970210-6
Date
1996
Source
Development (Cambridge, England)   123: 67-80 (Journal)
Registered Authors
Abdelilah-Seyfried, Salim, Driever, Wolfgang, Malicki, Jarema, Mountcastle-Shah, Elizabeth, Neuhauss, Stephan, Schier, Alexander, Solnica-Krezel, Lilianna, Stainier, Didier, Stemple, Derek L.
Keywords
organizer; gastrulation; epiboly; convergence; extension; zebrafish; dorsoventral polarity
MeSH Terms
  • Animals
  • Genetic Complementation Test
  • Gene Expression Regulation, Developmental*
  • Gastrula/cytology*
  • Gastrula/physiology*
  • Cell Survival/genetics
  • Zebrafish/anatomy & histology
  • Zebrafish/embryology*
  • Zebrafish/genetics*
  • DNA Mutational Analysis
  • Mutagenesis*
  • Phenotype
(all 12)
PubMed
9007230 Full text @ Development
Abstract
One of the major challenges of developmental biology is understanding the inductive and morphogenetic processes that shape the vertebrate embryo. In a large-scale genetic screen for zygotic effect, embryonic lethal mutations in zebrafish we have identified 25 mutations that affect specification of cell fates and/or cellular rearrangements during gastrulation. These mutations define at least 14 complementation groups, four of which correspond to previously identified genes. Phenotypic analysis of the ten novel loci revealed three groups of mutations causing distinct effects on cell fates in the gastrula. One group comprises mutations that lead to deficiencies in dorsal mesodermal fates and affect central nervous system patterning. Mutations from the second group affect formation of ventroposterior embryonic structures. We suggest that mutations in these two groups identify genes necessary for the formation, maintenance or function of the dorsal organizer and the ventral signaling pathway, respectively. Mutations in the third group affect primarily cellular rearrangements during gastrulation and have complex effects on cell fates in the embryo. This group, and to some extent mutations from the first two groups, affect the major morphogenetic processes, epiboly, convergence and extension, and tail morphogenesis. These mutations provide an approach to understanding the genetic control of gastrulation in vertebrates.
Genes / Markers
Figures
Figure Gallery (6 images)
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Expression
Phenotype
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
b180
    Deficiency
      m52
        Unknown
        m60
          Deficiency
          m70
            Unknown
            m100
              Point Mutation
              m101
                Unknown
                m119
                  Point Mutation
                  m122
                    Unknown
                    m134
                      Point Mutation
                      m144
                        Unknown
                        1 - 10 of 26
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                        Human Disease / Model
                        No data available
                        Sequence Targeting Reagents
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                        Fish
                        Antibodies
                        No data available
                        Orthology
                        No data available
                        Engineered Foreign Genes
                        No data available
                        Mapping
                        No data available