PUBLICATION

Mutations affecting cell fates and cellular rearrangements during gastrulation in zebrafish

Authors

Solnica-Krezel, L., Stemple, D.L., Mountcastle-Shah, E., Rangini, Z., Neuhauss, S.C., Malicki, J., Schier, A.F., Stainier, D.Y., Zwartkruis, F., Abdelilah, S., and Driever, W.

ID

ZDB-PUB-970210-6

Date

1996

Source

Development (Cambridge, England) 123: 67-80 (Journal)

Registered Authors

Abdelilah-Seyfried, Salim, Driever, Wolfgang, Malicki, Jarema, Mountcastle-Shah, Elizabeth, Neuhauss, Stephan, Schier, Alexander, Solnica-Krezel, Lilianna, Stainier, Didier, Stemple, Derek L.

Keywords

organizer; gastrulation; epiboly; convergence; extension; zebrafish; dorsoventral polarity

MeSH Terms

Animals
Genetic Complementation Test
Gene Expression Regulation, Developmental*
Gastrula/cytology*
Gastrula/physiology*
Cell Survival/genetics
Zebrafish/anatomy & histology
Zebrafish/embryology*
Zebrafish/genetics*
DNA Mutational Analysis
Mutagenesis*
Phenotype

(all 12)

PubMed

9007230 Full text @ Development

Abstract

One of the major challenges of developmental biology is understanding the inductive and morphogenetic processes that shape the vertebrate embryo. In a large-scale genetic screen for zygotic effect, embryonic lethal mutations in zebrafish we have identified 25 mutations that affect specification of cell fates and/or cellular rearrangements during gastrulation. These mutations define at least 14 complementation groups, four of which correspond to previously identified genes. Phenotypic analysis of the ten novel loci revealed three groups of mutations causing distinct effects on cell fates in the gastrula. One group comprises mutations that lead to deficiencies in dorsal mesodermal fates and affect central nervous system patterning. Mutations from the second group affect formation of ventroposterior embryonic structures. We suggest that mutations in these two groups identify genes necessary for the formation, maintenance or function of the dorsal organizer and the ventral signaling pathway, respectively. Mutations in the third group affect primarily cellular rearrangements during gastrulation and have complex effects on cell fates in the embryo. This group, and to some extent mutations from the first two groups, affect the major morphogenetic processes, epiboly, convergence and extension, and tail morphogenesis. These mutations provide an approach to understanding the genetic control of gastrulation in vertebrates.

Genes / Markers

Figures

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Expression

Gene	Fish	Conditions	Stage	Qualifier	Anatomy	Assay	Figure
dbx1a	dharma^m168/m168 (AB)	standard conditions	Prim-15	Not Detected	diencephalon	ISH	Fig. 2
dbx1a	dharma^m168/m168 (AB)	standard conditions	Prim-15		midbrain	ISH	Fig. 2
dbx1a	vangl2^m209/m209 (AB)	standard conditions	Bud		head	ISH	Fig. 9
dbx1a	WT	standard conditions	Prim-15		diencephalon	ISH	Fig. 2
dbx1a	WT	standard conditions	Bud		head	ISH	Fig. 9
dbx1a	WT	standard conditions	Prim-15		midbrain	ISH	Fig. 2
eve1	Df(Chr25:szl)m60/m60 (HK)	standard conditions	10-13 somites		tail bud	ISH	Fig. 7
eve1	gpc4^m119/m119 (AB)	standard conditions	Prim-5		post-vent region	ISH	Fig. 9
eve1	smad5^m169/m169 (AB)	standard conditions	10-13 somites to 14-19 somites		tail bud	ISH	Fig. 6
eve1	WT	standard conditions	Prim-5		post-vent region	ISH	Fig. 9

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Phenotype

Fish	Conditions	Stage	Phenotype	Figure
Df(Chr25:szl)m60/m60 (HK)	standard conditions	Bud	axis decreased length, abnormal	Fig. 3
Df(Chr25:szl)m60/m60 (HK)	standard conditions	Bud	convergent extension involved in axis elongation disrupted, abnormal	Fig. 3
Df(Chr25:szl)m60/m60 (HK)	standard conditions	Bud	tail bud decreased size, abnormal	Fig. 3
Df(Chr25:szl)m60/m60 (HK)	standard conditions	5-9 somites	axis decreased length, abnormal	Fig. 4
Df(Chr25:szl)m60/m60 (HK)	standard conditions	5-9 somites	mediolateral intercalation process quality, abnormal	Fig. 4
Df(Chr25:szl)m60/m60 (HK)	standard conditions	5-9 somites	post-vent region cell mislocalised, abnormal	Fig. 4
Df(Chr25:szl)m60/m60 (HK)	standard conditions	5-9 somites	tail bud shape, abnormal	Fig. 4
Df(Chr25:szl)m60/m60 (HK)	standard conditions	10-13 somites	mediolateral intercalation process quality, abnormal	Fig. 7
Df(Chr25:szl)m60/m60 (HK)	standard conditions	10-13 somites	somite anterior-posterior axis decreased height, abnormal	Fig. 7
Df(Chr25:szl)m60/m60 (HK)	standard conditions	10-13 somites	tail bud ventral region apoptotic, abnormal	Fig. 7

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Mutations / Transgenics

Allele	Type	Affected Genomic Region
b180	Deficiency
m52	Unknown	chrd
m60	Deficiency	szl
m70	Unknown	cpt
m100	Point Mutation	acvr1l
m101	Unknown	ndr2
m119	Point Mutation	gpc4
m122	Unknown	ndr2
m134	Point Mutation	tdgf1
m144	Unknown	vangl2

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Human Disease / Model

Sequence Targeting Reagents

Fish

Fish
acvr1l^m100/m100 (AB)
chrd^m52/m52 (AB)
chrd^m52 (AB)
cpt^m70/m70 (AB)
cpt^m282/m282
cpt^m346/m346 (AB)
cpt^m586/m586 (AB)
Df(Chr25:ogo_b180)b180/b180 (AB)
Df(Chr25:szl)m60/m60 (HK)
dharma^m168/m168 (AB)

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Antibodies

Orthology

Engineered Foreign Genes

Mapping

Solnica-Krezel et al., 1996 ZDB-PUB-970210-6 PMID:9007230

Mutations affecting cell fates and cellular rearrangements during gastrulation in zebrafish

Solnica-Krezel et al., 1996

ZDB-PUB-970210-6
PMID:9007230