PUBLICATION
Establishment of a zebrafish hematological disease model induced by 1,4-benzoquinone
- Authors
- Zhang, A., Wu, M., Tan, J., Yu, N., Xu, M., Yu, X., Liu, W., Zhang, Y.
- ID
- ZDB-PUB-190323-2
- Date
- 2019
- Source
- Disease models & mechanisms 12(3): (Journal)
- Registered Authors
- Wu, Mei, Xu, Mengchang, Zhang, Yiyue
- Keywords
- 1,4-benzoquinone, Hematotoxicity, Neutrophilia, Zebrafish, c-myb
- MeSH Terms
-
- Hematopoiesis/drug effects
- Proto-Oncogene Proteins c-myb/metabolism
- Kaplan-Meier Estimate
- Teratogens/toxicity
- Mutation/genetics
- Biomarkers/metabolism
- Myeloid Cells/drug effects
- Myeloid Cells/metabolism
- Benzoquinones/toxicity*
- Embryo, Nonmammalian/cytology
- Embryo, Nonmammalian/drug effects
- Embryo, Nonmammalian/metabolism
- Animals
- Disease Models, Animal
- Zebrafish/embryology
- Zebrafish/metabolism*
- Neutrophils/metabolism
- Neutrophils/pathology
- Hematologic Diseases/chemically induced*
- Hematologic Diseases/pathology*
- PubMed
- 30898970 Full text @ Dis. Model. Mech.
Citation
Zhang, A., Wu, M., Tan, J., Yu, N., Xu, M., Yu, X., Liu, W., Zhang, Y. (2019) Establishment of a zebrafish hematological disease model induced by 1,4-benzoquinone. Disease models & mechanisms. 12(3):.
Abstract
Benzene exposure is associated with various hematological disorders, especially leukemia. The reactive metabolite of benzene, 1,4-Benzoquinone (BQ), generated in bone marrow (BM), is suggested to be a key molecule in mediating benzene-induced hematotoxicity and carcinogenicity. Yet, its pathogenic role remains largely unknown due to lack of suitable vertebrate whole-organism models. Here, we present an in vivo study to reveal the effect of BQ exposure on hematotoxicity in zebrafish. From embryonic stages to adulthood, BQ exposure suppressed erythroid and lymphoid hematopoiesis but abnormally accumulated myeloid cells and precursors, which resembles benzene-induced cytopenia and myeloid dysplasia in humans. This myeloid expansion is caused by granulocyte but not macrophage lineage, emphasizing the significant role of lineage specificity in BQ-mediated hematopoietic toxicity. Analysis of the c-myb-deficient mutant cmybhkz3 revealed that BQ induced neutrophilia in a c-myb-dependent manner, demonstrating that c-myb is a key intrinsic mediator of BQ hematotoxicity. Our study reveals that BQ causes lineage-specific hematotoxicity in zebrafish from embryonic stages to adulthood. Since c-myb is indispensable for BQ to induce neutrophilia, c-myb may serve as a potential drug target for reversing BQ hematotoxicity.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping