PUBLICATION

Spatiotemporal Coordination of FGF and Shh Signaling Underlies the Specification of Myoblasts in the Zebrafish Embryo

Authors
Yin, J., Lee, R., Ono, Y., Ingham, P.W., Saunders, T.E.
ID
ZDB-PUB-180927-10
Date
2018
Source
Developmental Cell   46: 735-750.e4 (Journal)
Registered Authors
Ingham, Philip, Lee, Raymond, Ono, Yosuke, Saunders, Timothy Edward
Keywords
FGF signaling, Sonic hedgehog, adaxial cells, fast muscle fibers, in vivo imaging, myogenesis, somite polarity, somite rotation, temporal regulation, zebrafish
MeSH Terms
  • Cell Lineage*
  • Cells, Cultured
  • Zebrafish/embryology*
  • Zebrafish/physiology
  • Zebrafish Proteins/metabolism*
  • Fibroblast Growth Factors/metabolism*
  • Muscle, Skeletal/embryology*
  • Hedgehog Proteins/metabolism*
  • Myoblasts/cytology*
  • Myoblasts/metabolism
  • Signal Transduction
  • Animals
  • Morphogenesis
  • Cell Differentiation
(all 14)
PubMed
30253169 Full text @ Dev. Cell
Abstract
Somitic cells give rise to a variety of cell types in response to Hh, BMP, and FGF signaling. Cell position within the developing zebrafish somite is highly dynamic: how, when, and where these signals specify cell fate is largely unknown. Combining four-dimensional imaging with pathway perturbations, we characterize the spatiotemporal specification and localization of somitic cells. Muscle formation is guided by highly orchestrated waves of cell specification. We find that FGF directly and indirectly controls the differentiation of fast and slow-twitch muscle lineages, respectively. FGF signaling imposes tight temporal control on Shh induction of slow muscles by regulating the time at which fast-twitch progenitors displace slow-twitch progenitors from contacting the Shh-secreting notochord. Further, we find a reciprocal regulation of fast and slow muscle differentiation, morphogenesis, and migration. In conclusion, robust cell fate determination in the developing somite requires precise spatiotemporal coordination between distinct cell lineages and signaling pathways.
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Phenotype
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
b641
    Point Mutation
    i106TgTransgenic Insertion
      i233TgTransgenic Insertion
        i316TgTransgenic Insertion
          pd1TgTransgenic Insertion
            pd3TgTransgenic Insertion
              ti1
                Small Deletion
                tp39
                  Point Mutation
                  1 - 8 of 8
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                  Human Disease / Model
                  No data available
                  Sequence Targeting Reagents
                  Target Reagent Reagent Type
                  ripply1MO1-ripply1MRPHLNO
                  1 - 1 of 1
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                  Fish
                  Antibodies
                  Name Type Antigen Genes Isotypes Host Organism
                  Ab1-engmonoclonalIgG1Mouse
                  Ab-Pax7monoclonal
                    IgG1Mouse
                    1 - 2 of 2
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                    Orthology
                    Engineered Foreign Genes
                    Marker Marker Type Name
                    DsRedEFGDsRed
                    EGFPEFGEGFP
                    GAL4EFGGAL4
                    1 - 3 of 3
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                    Mapping