PUBLICATION

Spatiotemporal Coordination of FGF and Shh Signaling Underlies the Specification of Myoblasts in the Zebrafish Embryo

Authors

Yin, J., Lee, R., Ono, Y., Ingham, P.W., Saunders, T.E.

ID

ZDB-PUB-180927-10

Date

2018

Source

Developmental Cell 46: 735-750.e4 (Journal)

Registered Authors

Ingham, Philip, Lee, Raymond, Ono, Yosuke, Saunders, Timothy Edward

Keywords

FGF signaling, Sonic hedgehog, adaxial cells, fast muscle fibers, in vivo imaging, myogenesis, somite polarity, somite rotation, temporal regulation, zebrafish

MeSH Terms

Zebrafish/embryology*
Zebrafish/physiology
Fibroblast Growth Factors/metabolism*
Animals
Muscle, Skeletal/embryology*
Zebrafish Proteins/metabolism*
Signal Transduction
Hedgehog Proteins/metabolism*
Cells, Cultured
Cell Lineage*
Morphogenesis
Cell Differentiation
Myoblasts/cytology*
Myoblasts/metabolism

(all 14)

PubMed

30253169 Full text @ Dev. Cell

Abstract

Somitic cells give rise to a variety of cell types in response to Hh, BMP, and FGF signaling. Cell position within the developing zebrafish somite is highly dynamic: how, when, and where these signals specify cell fate is largely unknown. Combining four-dimensional imaging with pathway perturbations, we characterize the spatiotemporal specification and localization of somitic cells. Muscle formation is guided by highly orchestrated waves of cell specification. We find that FGF directly and indirectly controls the differentiation of fast and slow-twitch muscle lineages, respectively. FGF signaling imposes tight temporal control on Shh induction of slow muscles by regulating the time at which fast-twitch progenitors displace slow-twitch progenitors from contacting the Shh-secreting notochord. Further, we find a reciprocal regulation of fast and slow muscle differentiation, morphogenesis, and migration. In conclusion, robust cell fate determination in the developing somite requires precise spatiotemporal coordination between distinct cell lineages and signaling pathways.

Genes / Markers

Figures

Show all Figures

Expression

Gene	Fish	Conditions	Stage	Anatomy	Assay	Figure
EGFP	i106Tg	control	14-19 somites	slow muscle cell	IHC	Fig. 3
EGFP	i106Tg	control	Prim-5	slow muscle cell	IHC	Fig. 3
EGFP	i106Tg	chemical treatment by environment: SU5402	Prim-5	slow muscle cell	IHC	Fig. 3
EGFP	i106Tg; pd1Tg	heat shock	Prim-5	slow muscle cell	IHC	Fig. 3
EGFP	i106Tg; pd3Tg	heat shock	Prim-5	slow muscle cell	IHC	Fig. 3
EGFP	i233Tg	control	Prim-5	muscle pioneer	IHC	Fig. 3
EGFP	i233Tg	chemical treatment by environment: SU5402	Prim-5	muscle pioneer	IHC	Fig. 3
EGFP	i233Tg; pd1Tg	heat shock	Prim-5	muscle pioneer	IHC	Fig. 3
EGFP	i233Tg; pd3Tg	heat shock	Prim-5	muscle pioneer	IHC	Fig. 3
etv4	i106Tg	control	14-19 somites	somite anterior region	ISH	Fig. 3

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Phenotype

Fish	Conditions	Stage	Phenotype	Figure
i106Tg	chemical treatment by environment: SU5402	Prim-5	skeletal muscle satellite cell increased amount, abnormal	Fig. 3
i106Tg + MO1-ripply1	control	Prim-15	fast muscle cell skeletal muscle tissue development decreased occurrence, abnormal	Fig. 7
i106Tg + MO1-ripply1	control	Prim-15	slow muscle cell muscle cell migration decreased occurrence, abnormal	Fig. 7
i106Tg; pd1Tg	heat shock	Prim-5	skeletal muscle satellite cell increased amount, abnormal	Fig. 3
i106Tg; pd3Tg	heat shock	Prim-5	skeletal muscle satellite cell decreased amount, abnormal	Fig. 3
i233Tg	chemical treatment by environment: SU5402	Prim-5	muscle pioneer increased amount, abnormal	Fig. 3
i233Tg + MO1-ripply1	control	Prim-15	fast muscle cell skeletal muscle tissue development decreased occurrence, abnormal	Fig. 7
i233Tg + MO1-ripply1	control	Prim-15	skeletal muscle satellite cell mislocalised, abnormal	Fig. 7
i233Tg; pd1Tg	heat shock	Prim-5	muscle pioneer increased amount, abnormal	Fig. 3
i233Tg; pd3Tg	heat shock	Prim-5	muscle pioneer decreased amount, abnormal	Fig. 3

1 - 10 of 15

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Mutations / Transgenics

Allele	Construct	Type	Affected Genomic Region
b641		Point Mutation	smo
i106Tg	TgPAC(prdm1a:EGFP)	Transgenic Insertion
i233Tg	Tg(-10en2a:EGFP)	Transgenic Insertion
i316Tg	Tg(smyhc1:GAL4-VP16)	Transgenic Insertion
pd1Tg	Tg(hsp70l:dnfgfr1a-EGFP)	Transgenic Insertion
pd3Tg	Tg(hsp70l:Xla.Fgfr1,cryaa:DsRed)	Transgenic Insertion
ti1		Small Deletion	tbx6
tp39		Point Mutation	prdm1a

1 - 8 of 8

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Human Disease / Model

Sequence Targeting Reagents

Target	Reagent	Reagent Type
ripply1	MO1-ripply1	MRPHLNO

Fish

Fish
i106Tg
i106Tg + MO1-ripply1
i106Tg; pd1Tg
i106Tg; pd3Tg
i233Tg
i233Tg + MO1-ripply1
i233Tg; pd1Tg
i233Tg; pd3Tg
prdm1a^tp39/tp39; i106Tg
smo^b641/b641

Antibodies

Name	Type	Antigen Genes	Isotypes	Host Organism
Ab1-eng	monoclonal	en1a en1b en2a en2b	IgG1	Mouse
Ab-Pax7	monoclonal		IgG1	Mouse

Orthology

Engineered Foreign Genes

Marker	Marker Type	Name
DsRed	EFG	DsRed
EGFP	EFG	EGFP
GAL4	EFG	GAL4

Mapping

Yin et al., 2018 ZDB-PUB-180927-10 PMID:30253169

Spatiotemporal Coordination of FGF and Shh Signaling Underlies the Specification of Myoblasts in the Zebrafish Embryo

Yin et al., 2018

ZDB-PUB-180927-10
PMID:30253169