PUBLICATION

sox2 and sox3 cooperate to regulate otic/epibranchial placode induction in zebrafish

Authors

Gou, Y., Guo, J., Maulding, K., Riley, B.B.

ID

ZDB-PUB-180123-4

Date

2018

Source

Developmental Biology 435(1): 84-95 (Journal)

Registered Authors

Riley, Bruce

Keywords

CRISPR/Cas9, SoxB1, TALEN, pluripotency, progenitors

MeSH Terms

SOX Transcription Factors/genetics
SOX Transcription Factors/metabolism*
Organogenesis/physiology*
PAX8 Transcription Factor/genetics
PAX8 Transcription Factor/metabolism
Mutation*
Ear, Inner/embryology*
Animals
Zebrafish/embryology*
Zebrafish/genetics
Zebrafish Proteins/genetics
Zebrafish Proteins/metabolism*

(all 12)

PubMed

29355522 Full text @ Dev. Biol.

Abstract

Expression of sox3 is one of the earliest markers of Fgf-dependent otic/epibranchial placode induction. We report here that sox2 is also expressed in the early otic/epibranchial placode in zebrafish. To address functions of sox2 and sox3, we generated knockouts and heat shock-inducible transgenes. Mutant analysis, and low-level misexpression, showed that sox2 and sox3 act redundantly to establish a full complement of otic/epibranchial cells. Disruption of pax8, another early regulator, caused similar placodal deficiencies to sox3 mutants or pax8-sox3 double mutants, suggesting that sox3 and pax8 operate in the same pathway. High-level misexpression of sox2 or sox3 during early stages cell-autonomously blocked placode induction, whereas misexpression several hours later could not reverse placodal differentiation. In an assay for ectopic placode-induction, we previously showed that misexpression of fgf8 induces a high level of ectopic sox3, but not pax8. Partial knockdown of sox3 significantly enhanced ectopic induction of pax8, whereas full knockdown of sox3 inhibited this process. Together these findings show that sox2 and sox3 are together required for proper otic induction, but the level of expression must be tightly regulated to avoid suppression of differentiation and maintenance of pluripotency.

Genes / Markers

Figures

Show all Figures

Expression

Gene	Fish	Conditions	Stage	Anatomy	Assay	Figure
cldna	AB	control	Prim-5	otic vesicle	ISH	Fig. 6
cldna	pax2a^tu29a/tu29a (AB)	standard conditions	Prim-5	otic vesicle	ISH	Fig. 6
cldna	pax2a^tu29a/tu29a; pax8^{nia03Gt/nia03Gt} (AB)	standard conditions	Prim-5	otic vesicle	ISH	Fig. 6
myod1	AB	control	10-13 somites	somite	ISH	Fig. 8
myod1	AB	control	5-9 somites	somite	ISH	Fig. 6
myod1	AB	control	5-9 somites	somite	ISH	Fig. 7
myod1	AB	control	5-9 somites	somite	ISH	Fig. 8
myod1	AB + MO3-sox3 + MO4-tp53	standard conditions	5-9 somites	somite	ISH	Fig. 7
myod1	pax2a^tu29a/tu29a (AB)	standard conditions	5-9 somites	somite	ISH	Fig. 6
myod1	pax2a^tu29a/tu29a; pax8^{nia03Gt/nia03Gt} (AB)	standard conditions	5-9 somites	somite	ISH	Fig. 6

1 - 10 of 76

Show

Phenotype

Fish	Conditions	Stage	Phenotype	Figure
AB + MO3-sox3 + MO4-tp53	standard conditions	5-9 somites	otic placode pax2a expression decreased amount, abnormal	Fig. 7
AB + MO3-sox3 + MO4-tp53	standard conditions	5-9 somites	otic placode pax2a expression decreased distribution, abnormal	Fig. 7
pax2a^tu29a/tu29a (AB)	standard conditions	5-9 somites	otic placode pax2a expression decreased amount, abnormal	Fig. 6
pax2a^tu29a/tu29a (AB)	standard conditions	5-9 somites	otic placode pax2a expression decreased distribution, abnormal	Fig. 6
pax2a^tu29a/tu29a (AB)	standard conditions	26+ somites	otic vesicle pax2a expression decreased amount, abnormal	Fig. 6
pax2a^tu29a/tu29a (AB)	standard conditions	26+ somites	otic vesicle pax2a expression decreased distribution, abnormal	Fig. 6
pax2a^tu29a/tu29a; pax8^{nia03Gt/nia03Gt} (AB)	standard conditions	5-9 somites	otic placode pax2a expression decreased amount, abnormal	Fig. 6
pax2a^tu29a/tu29a; pax8^{nia03Gt/nia03Gt} (AB)	standard conditions	5-9 somites	otic placode pax2a expression decreased distribution, abnormal	Fig. 6
pax2a^tu29a/tu29a; pax8^{nia03Gt/nia03Gt} (AB)	standard conditions	5-9 somites	otic placode decreased size, abnormal	Fig. 6
pax2a^tu29a/tu29a; pax8^{nia03Gt/nia03Gt} (AB)	standard conditions	5-9 somites	otic placode morphology, abnormal	Fig. 6

1 - 10 of 77

Show

Mutations / Transgenics

Allele	Construct	Type	Affected Genomic Region
nia03Gt	Gt(pax8:DsRedx)	Transgenic Insertion	pax8
s356tTg	Tg(pou4f3:GAP-GFP)	Transgenic Insertion
tu29a		Point Mutation	pax2a
x17Tg	Tg1(hsp70l:fgf8a)	Transgenic Insertion
x21Tg	Tg(hsp70l:sox2)	Transgenic Insertion
x32Tg	Tg(hsp70l:sox3)	Transgenic Insertion
x50		Small Deletion	sox2
x52		Small Deletion	sox3
x53		Indel	sox3

1 - 9 of 9

Show

Human Disease / Model

Sequence Targeting Reagents

Target	Reagent	Reagent Type
sox2	TALEN2-sox2	TALEN
sox3	CRISPR1-sox3	CRISPR
sox3	MO3-sox3	MRPHLNO
tp53	MO4-tp53	MRPHLNO

Fish

Fish
AB
AB + MO3-sox3 + MO4-tp53
pax2a^tu29a/tu29a (AB)
pax2a^tu29a/tu29a; pax8^{nia03Gt/nia03Gt} (AB)
pax8^{nia03Gt/nia03Gt} (AB)
sox2^x50/x50 (AB)
sox2^x50/x50; sox3^x52/x52 (AB)
sox3^x52/x52 (AB)
sox3^x52/x52; pax8^{nia03Gt/nia03Gt} (AB)
x17Tg/+ (AB)

1 - 10 of 15

Show

Antibodies

Orthology

Engineered Foreign Genes

Marker	Marker Type	Name
DsRedx	EFG	DsRedx
GFP	EFG	GFP

Mapping

Gou et al., 2018 ZDB-PUB-180123-4 PMID:29355522

sox2 and sox3 cooperate to regulate otic/epibranchial placode induction in zebrafish

Gou et al., 2018

ZDB-PUB-180123-4
PMID:29355522