PUBLICATION

Glypican4 Modulates Lateral Line Collective Cell Migration Non Cell-Autonomously

Authors
Venero Galanternik M., Lush, M.E., Piotrowski, T.
ID
ZDB-PUB-160920-9
Date
2016
Source
Developmental Biology   419(2): 321-335 (Journal)
Registered Authors
Lush, Mark E., Piotrowski, Tatjana, Venero Galanternik, Marina
Keywords
FGF, Heparinase III, Wnt/β-catenin, boxer, dackel, extl3/ext2, lamininγ1, lateral line, morphogenesis, primordium, proteoglycans, sulf1
MeSH Terms
  • Animals
  • Zebrafish/embryology
  • Gene Expression Regulation, Developmental
  • Feedback, Physiological
  • Gastrula/physiology
  • Cell Polarity
  • Muscle Development/physiology
  • Ectoderm/cytology
  • Ectoderm/physiology
  • Ectoderm/transplantation
  • Lateral Line System/cytology
  • Lateral Line System/embryology*
  • Wnt Signaling Pathway/physiology
  • Muscle, Skeletal/embryology
  • Zebrafish Proteins/physiology*
  • Bone Morphogenetic Proteins/physiology
  • Cell Movement
  • Hedgehog Proteins/physiology
  • Heparan Sulfate Proteoglycans/physiology*
  • Glypicans/genetics
  • Glypicans/physiology*
(all 21)
PubMed
27640326 Full text @ Dev. Biol.
Abstract
Collective cell migration is an essential process during embryonic development and diseases such as cancer, and still much remains to be learned about how cell intrinsic and environmental cues are coordinated to guide cells to their targets. The migration-dependent development of the zebrafish sensory lateral line proves to be an excellent model to study how proteoglycans control collective cell migration in a vertebrate. Proteoglycans are extracellular matrix glycoproteins essential for the control of several signaling pathways including Wnt/β-catenin, Fgf, BMP and Hh. In the lateral line primordium the modified sugar chains on proteoglycans are important regulators of cell polarity, ligand distribution and Fgf signaling. At least five proteoglycans show distinct expression patterns in the primordium; however, their individual functions have not been studied. Here, we describe the function of glypican4 during zebrafish lateral line development. glypican4 is expressed in neuromasts, interneuromast cells and muscle cells underlying the lateral line. knypekfr6/glypican4 mutants show severe primordium migration defects and the primordium often U-turns and migrates back toward the head. Our analysis shows that Glypican4 regulates the feedback loop between Wnt/β-catenin/Fgf signaling in the primordium redundantly with other Heparan Sulfate Proteoglycans. In addition, the primordium migration defect is caused non-cell autonomously by the loss of cxcl12a-expressing muscle precursors along the myoseptum via downregulation of Hh. Our results show that glypican4 has distinct functions in primordium cells and cells in the environment and that both of these functions are essential for collective cell migration.
Genes / Markers
Figures
Figure Gallery (14 images) / 2
Show all Figures
Expression
Phenotype
Fish Conditions Stage Phenotype Figure
WTchemical treatment by environment: dorsomorphinLong-pec
WTchemical treatment by environment: dorsomorphinLong-pec
gpc4fr6/+chemical treatment by environment: sodium chlorateHigh-pec
gpc4fr6/fr6standard conditionsPrim-5
gpc4fr6/fr6standard conditionsPrim-5
gpc4fr6/fr6standard conditionsPrim-5
gpc4fr6/fr6standard conditionsPrim-5
gpc4fr6/fr6standard conditionsPrim-5
gpc4fr6/fr6standard conditionsPrim-5
gpc4fr6/fr6standard conditionsPrim-15
1 - 10 of 28
Show
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
a131TgTransgenic Insertion
    fr6
      		Point Mutation
      kyu1TgTransgenic Insertion
        psi4TgTransgenic Insertion
          t30516
            		Point Mutation
            w32TgTransgenic Insertion
              zf106TgTransgenic Insertion
                1 - 7 of 7
                Show
                Human Disease / Model
                No data available
                Sequence Targeting Reagents
                No data available
                Fish
                Fish
                gpc4fr6/fr6
                gpc4fr6/fr6; w32Tg
                gpc4fr6/fr6; zf106Tg
                gpc4fr6/+
                w32Tg
                WT
                1 - 6 of 6
                Show
                Antibodies
                Orthology
                No data available
                Engineered Foreign Genes
                Marker Marker Type Name
                CFPEFGCFP
                d2EGFPEFGd2EGFP
                EGFPEFGEGFP
                GFPEFGGFP
                mCherryEFGmCherry
                RFPEFGRFP
                YFPEFGYFP
                1 - 7 of 7
                Show
                Mapping
                No data available
                Your Input Welcome
                We welcome your input and comments. Please use this form to recommend updates to the information in ZFIN. We appreciate as much detail as possible and references as appropriate. We will review your comments promptly.
                Citation
                Venero Galanternik M., Lush, M.E., Piotrowski, T. (2016) Glypican4 Modulates Lateral Line Collective Cell Migration Non Cell-Autonomously. Developmental Biology. 419(2):321-335.